Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing total salvianolic acid

A technology for total phenolic acid and temperature control of Salvia miltiorrhiza, applied in the field of medicine, can solve the problems of loss of curative effect, difficulty, low industrial production cost, etc., and achieve the effects of avoiding the loss of phenolic acid active ingredients, reducing the loss of active ingredients and optimizing production conditions.

Active Publication Date: 2012-11-21
CHENGDU DIAO JIU HONG PHARMACEUTICAL FACTORY
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some problems in the preparation of salvia miltiorrhiza by the above methods, which affect the yield of industrialization and finished products.
First of all, most of the above methods are extracted by boiling water or heating for a long time (50-60°C), and salvianolic acid is a polyphenol structure, which is easily oxidized and degraded by heat, and loses its curative effect, thus seriously affecting the quality of the product. quality and curative effect; secondly, most extraction methods adopt the step of high-concentration ethanol precipitation, which greatly reduces the safety of industrial production, the consumption of ethanol is large, and the production cost is high; thirdly, when the above-mentioned method is extracted, a large amount of concentrated water is needed, It is not convenient for industrialized large-scale production, and the operation is relatively complicated; the fourth, the yield of salvianolic acid extracted by the above-mentioned method is low, generally 2 to 4%, and the low yield also increases the cost of industrialized production and brings difficulties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing total salvianolic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Extracting Salvia phenolic acids from the traditional Chinese medicine Salvia miltiorrhiza

[0025] Salvia miltiorrhiza 5kg, softened by steam. Cut into pieces, add 25L of deionized water, cold soak for 6h, percolate for 24h, flow rate 2L / h. Collect 45L of percolation liquid, centrifuge, put on macroporous adsorption resin column HPD100, elute with 7.5L of water, add 20L of 70% ethanol to elute, collect ethanol eluate, dry under reduced pressure, temperature is controlled at 60°C. Obtain salvianolic acid dry powder 320g, finished product yield 6.4%. After testing, the salvianolic acid B content was 71.2%, and the total phenolic acid content was 90.4%.

[0026] Detection method of salvianolic acid B and total salvianolic acid:

[0027] (1) Salvianolic acid B: determined by HPLC method, the detection wavelength is 285nm, and the standard salvianolic acid B is provided by China Institute for the Control of Pharmaceutical and Biological Products, with a purity ...

Embodiment 2

[0031] Example 2 Extracting total phenolic acids of Salvia miltiorrhiza from Chinese medicine Salvia miltiorrhiza

[0032] Salvia miltiorrhiza 5kg, softened by steam. Cut into pieces, add 25L of deionized water, cold soak for 24h, percolate for 18h, flow rate 2L / h. Collect 45L of the percolation solution, centrifuge, put it on a macroporous adsorption resin column HPD100, elute with 7.5L of water, then add 20L of 95% ethanol, collect the ethanol eluate, dry it under reduced pressure, and control the temperature at 60°C. Obtain salvianolic acid dry powder 300g, finished product yield 6%. Measured by the method of Example 1, the salvianolic acid B content was 62.7%, and the total phenolic acid content was 86.4%.

Embodiment 3

[0033] Example 3 Extracting the total phenolic acid of Salvia miltiorrhiza from Chinese medicine Salvia miltiorrhiza

[0034] Salvia miltiorrhiza 5kg, softened by steam. Cut into pieces, add 25L of deionized water, cold soak for 12h, percolate for 12h, flow rate 2L / h. Collect 45L of percolation solution, centrifuge, put on macroporous adsorption resin column HPD100, elute with 7.5L of water, add 20L of 80% ethanol to elute, collect ethanol eluate, dry under reduced pressure, the temperature is controlled at 60°C. Obtain salvianolic acid dry powder 275g, finished product yield 5.5%. Measured by the method of Example 1, the salvianolic acid B content is 61.5%, and the total phenolic acid content is 82.5%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing total salvianolic acid and application thereof. The preparation method comprises the following steps of: impregnating root of red-rooted salvia by cold water, extracting by a diacolation process, adsorbing diacolation liquid by macroporous adsorbent resin, eluting, concentrating and drying eluent under reduced pressure to obtain the total salvianolicacid. The method for preparing the total salvianolic acid has the advantages and characteristics of simple steps, convenience for operation, high yield of finished products (which is over 5 percent generally), less energy consumption, no pollution, high safety and easiness in industrial production. The obtained total salvianolic acid can be used as medicaments for preventing and treating cardiovascular and cerebrovascular diseases and the like.

Description

[0001] The present invention is a divisional application, the original application number is 200510020270.X, the original application date is January 28, 2005, and the original application name is: preparation method of salvia miltiorrhiza total phenolic acid. technical field [0002] The invention relates to a preparation method of a traditional Chinese medicine extract, in particular to a method for extracting salvia miltiorrhiza from the traditional Chinese medicine Salvia miltiorrhiza, belonging to the technical field of medicine. Background technique [0003] Salvia miltiorrhiza is the dry root and rhizome of Salvia salviz miltiorrhiza Bge., a plant of the Labiatae family. It has the effects of promoting blood circulation and removing blood stasis, cooling blood and eliminating carbuncle, eliminating troubles and calming the nerves. Modern pharmacological studies have confirmed that Danshen has the effects of dilating blood vessels, improving microcirculation, increasing...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/537A61P9/10A61K125/00
Inventor 付铁军李伯刚
Owner CHENGDU DIAO JIU HONG PHARMACEUTICAL FACTORY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com