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Method for increasing Gluconobacter oxydans biomass by fermenting with oxygen-carrier-added culture medium

A technology for oxidizing glucose and fermentation methods, applied in microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problems of decreased activity, methods of fermenting and increasing the biomass of Gluconobacter oxydans, and the like. Enhanced biocatalytic ability, less foam formation, and less shear force

Active Publication Date: 2015-07-22
LUNAN PHARMA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has considerable randomness, and if the strain is treated with mutagen for a long time, its viability will gradually decrease
[0006] At present, there is no relevant literature report on the method of increasing the biomass of Gluconobacter oxydans by fermenting the medium with the addition of oxygen carrier

Method used

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  • Method for increasing Gluconobacter oxydans biomass by fermenting with oxygen-carrier-added culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0022] (1) Bacteria culture

[0023] Inoculate the strain of Gluconobacter oxydans into the seed medium with a pH of 7.0, and cultivate it in a constant temperature shaking incubator at 25° C. at 150 rpm for 25 hours. The composition and content of the seed medium are: sorbitol 50g / L, yeast extract 20g / L, potassium dihydrogen phosphate 0.5g / L.

[0024] (2) Fermentation culture

[0025] Inoculate the seed culture solution obtained through cultivation into the fermentation medium, the inoculum size is 5%, the composition and content of the fermentation medium are: sorbitol 50g / L, yeast extract 20g / L, potassium dihydrogen phosphate 1g / L, The pH is 7.0. The fermentation culture method adopts shake flask culture, that is, a 500ml Erlenmeyer flask with a liquid volume of 50ml is used. After 40 hours of culture in a constant temperature shaking incubator at 25°C and 200rpm, it is centrifuged at 10,000rpm for 15 minutes in a high-speed refrigerated centrifuge to collect bacteria. 4...

Embodiment 2

[0029] (1) Bacteria culture

[0030] Inoculate the strain of Gluconobacter oxydans into the seed medium with a pH of 7.0, and cultivate it in a constant temperature shaking incubator at 25° C. at 150 rpm for 25 hours. The composition and content of the seed medium are: sorbitol 50g / L, yeast extract 20g / L, potassium dihydrogen phosphate 0.5g / L.

[0031] (2) Fermentation culture

[0032] Inoculate the seed culture solution obtained through cultivation into the fermentation medium, the inoculum size is 5%, the composition and content of the fermentation medium are: sorbitol 50g / L, yeast extract 20g / L, potassium dihydrogen phosphate 1g / L, 20ml / tween-80L filter-sterilized n-hexane, pH 7.0. The fermentation culture method adopts shake flask culture, that is, a 500ml Erlenmeyer flask with a liquid volume of 50ml is used. After 40 hours of culture in a constant temperature shaking incubator at 25°C and 200rpm, it is centrifuged at 10,000rpm for 15 minutes in a high-speed refrigerate...

Embodiment 3

[0036] (1) Bacteria culture

[0037] Inoculate the strain of Gluconobacter oxydans into the seed medium with a pH of 7.0, and cultivate for 20 hours at 30° C. in a constant temperature shaking incubator at 200 rpm. The composition and content of the seed medium are: sorbitol 80g / L, yeast extract 20g / L, potassium dihydrogen phosphate 2g / L.

[0038] (2) Fermentation culture

[0039] Inoculate the seed culture solution obtained through cultivation into the fermentation medium, the inoculum size is 5%, the composition and content of the fermentation medium are: sorbitol 60g / L, yeast extract 20g / L, potassium dihydrogen phosphate 1g / L, 30ml / L filter-sterilized n-hexane, pH 7.0. The fermentation culture method adopts shake flask culture, that is, a 500 ml Erlenmeyer flask with a liquid volume of 50 ml is cultivated in a constant temperature shaking incubator at 30°C and 220 rpm for 48 hours, and then centrifuged at 10,000 rpm for 15 minutes in a high-speed refrigerated centrifuge t...

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Abstract

The invention relates to a method for increasing Gluconobacter oxydans biomass by fermenting with an oxygen-carrier-added culture medium. An appropriate oxygen carrier is added into a culture medium to provide an oxygen carrier fermenting system, so that the concentration of dissolved oxygen in the culture medium is greatly increased without adding other substances under the condition of no extra energy consumption, thereby greatly increasing the fermentation biomass.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for increasing the biomass of Gluconobacter oxydans by fermenting a culture medium added with an oxygen carrier. technical background [0002] Miglitol (migliton) is a new type of anti-diabetic drug launched by Bayer in 1997. It is a new type of intestinal α-glucosidase inhibitor discovered from the bacillus broth medium. It is the parent modified product of 1-deoxynojirimycin and belongs to the N-substituted-1-deoxynojirimycin type. Similar to glucose. Although Miglitol can be prepared by chemical synthesis, there are many steps in the preparation of Miglitol by chemical total synthesis, and the four chiral centers contained in the Miglitol molecule have also brought great challenges to chemical synthesis. Difficulties. Most of the current production scale adopts biosynthesis to prepare miglitol. The biosynthesis of miglitol generally has two routes ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/20C12P17/12C12R1/01
Inventor 赵志全王钦超肖月华
Owner LUNAN PHARMA GROUP CORPORATION
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