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Method for producing high-concentration ethanol by mixed fermentation of starch and molasses

A high-concentration, starch-based technology, applied in fermentation, biofuels, etc., to reduce the concentration of solids, weaken the toxic effect, and increase the concentration of ethanol

Inactive Publication Date: 2013-09-11
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few domestic studies on the fermentation of mixed raw materials. People such as Yi Yi have carried out research on the mixed fermentation of cassava flour and molasses to produce citric acid (Yi Yi et al. Research on mixed fermentation of cassava flour and molasses citric acid. Food Science, 2007, 28 (10 ): 395~397.); Chen Yong et al. ferment cassava flour sugarcane juice mixed raw material first saccharification and then fermentation (SHF) process (Chinese invention patent application, publication number CN 101070549A); and adopt synchronous saccharification with starchy and molasses raw materials (SSF) mixed fermentation to produce high-concentration fuel ethanol has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Fermentation test was conducted with Saccharomyces cerevisiae Y09tj selected by the applicant as a strain.

[0015] The bacteria has been deposited in the General Microbiology Center of the China Microbial Culture Collection Management Committee on November 24, 2009, and the preservation address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3 Beichen West Road, Chaoyang District, Beijing, and the preservation date is November 2009 On the 24th, the deposit number was CGMCC NO.3476.

[0016] Strain preservation medium (YPED): 20g glucose, 20g peptone, 10g yeast powder, 20g agar, distilled water to make the volume to 1000ml;

[0017] Seed Medium (YPD): 20g glucose, 20g peptone, 10g yeast powder, distilled water to make the volume to 1000ml;

[0018] Enzymes used: High temperature resistant amylase: Liquozyme Supra, purchased from Novozymes, with a standard enzyme activity of 90KNU / g. Glucoamylase: Dextrozyme DX, purchased from Novozymes, standard e...

Embodiment 2

[0043] Example 2: Fermentation with Saccharomyces cerevisiae (Saccharomyces cerevisiae) strain.

[0044] Yeast strain: Angel high temperature resistant active dry yeast, sold by Angel Yeast Co., Ltd.

[0045] Seed solution preparation: rehydrate the dry yeast with 35~40℃ 2% glucose solution for 15-20min, then lower the temperature to below 34℃ and activate for 1.5h, then it can be used as seed solution.

[0046] (1) Tapioca flour is used as the starch material, and the molasses material is added twice during the fermentation process

[0047] Take 30g of cassava flour and put it into a 250mL Erlenmeyer flask. The addition amount of urea is 0.25% (W / W), the addition amount of magnesium sulfate is 0.04% (W / W), and the addition amount is 0.15KNU / g high temperature α of cassava flour. -Amylase, add tap water to 100g, liquefy in a shaking water bath at 85°C for 0.5h, inoculate and ferment at 14 million cells / mL fermentation broth, and add 0.27AGU / g cassava powder glucoamylase, adjust the in...

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PUM

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Abstract

The invention discloses a method for producing high-concentration ethanol by the mixed fermentation of starch and molasses which are used as raw materials. The method comprises the following steps of: adding water to the raw material of starch and stirring to constant volume, liquefying at low temperature and simultaneously fermenting and saccharifying, and then adding the raw material of molasses for mixed fermentation through the batch or continuous flow feeding supplement way. The mixing ratio of the raw materials of starch and molasses is 1:2-8:1 (W / V), the temperature of fermentation is 30-37 DEG C, the time of fermentation lasts for 40-48h, the percentage of ethanol yield is 12.43-16.67% (V / V). The invention changes the traditional single raw material fermentation way, and not only improves the total sugar concentration of the fermented mash, but also reduces the viscosity of starch raw mash and improves the liquidity, so the concentration of ethanol in the fermentation mash is increased, and the raw material utilization rate is improved, and ethanol distillation energy consumption and production cost are decreased, the amount of sewage discharge is reduced, the load of sewage treatment is lowered and the ethanol production process is more economical and environmentally friendly.

Description

Technical field [0001] The present invention relates to the technical field of biological fermentation engineering, in particular to a method for fermenting high-concentration ethanol with starch and molasses mixed raw materials. Background technique [0002] The raw materials used in the production of ethanol and fuel ethanol in the prior art are basically a single raw material. For example, starch raw materials and molasses are common raw materials for ethanol production by fermentation. When starchy raw materials are used for thick mash fermentation, there are disadvantages of high solids concentration and poor fluidity, and precipitation is prone to occur, which is not conducive to continuous fermentation in large tanks, and is also not conducive to indirect heating of the reboiler of the mash tower during distillation. The waste grains are not easy to handle because of the high concentration of COD. Molasses contains 512% colloidal substances composed of pectin, caramel and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06
CPCY02E50/17Y02E50/10
Inventor 申乃坤黄日波王青艳陈东黄志民黎贞崇秦艳廖思明王成华陆雁金辉
Owner GUANGXI ACAD OF SCI
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