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Compound enzymatic hydrolysis method for straw raw materials

A compound enzymatic hydrolysis and raw material technology, applied in the field of compound enzymatic hydrolysis of straw raw materials, can solve the problems of affecting the output rate of ethanol fuel, high cost of fuel ethanol, and high cost of use, so as to achieve small investment, increase output rate, and output high effect

Inactive Publication Date: 2011-05-25
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of using enzymes in the above methods is high, and the use efficiency is low, which affects the output rate of ethanol fuel, so that the cost of producing fuel ethanol is too high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: The composite enzymatic hydrolysis method of straw raw materials, comprising the following steps: the straw raw materials are pulverized first, then adding water 5 times the weight of the straw raw materials to adjust the concentration, and adding concentrated hydrochloric acid so that the mass concentration of hydrochloric acid in the solution is 0.5% , hydrolyze for 2 hours to degrade lignin, then add xylanase, cellulase, amylase, pectinase and β-glucanase, use 0.2ml xylanase, cellulose Enzyme 2ml, amylase 1ml, pectinase 0.3g and β-glucanase 0.2g, continue to adjust the pH of the mixture to 4.3 with dilute hydrochloric acid, and then carry out enzymatic hydrolysis at 40°C for 72h.

Embodiment 2

[0014] Embodiment 2: the composite enzymatic hydrolysis method of straw raw materials, comprising the following steps: the straw raw materials are pulverized first, then adding water 10 times the weight of the straw raw materials to adjust the concentration, adding concentrated hydrochloric acid to make the mass concentration of hydrochloric acid in the solution be 1% , hydrolyze for 1.5 hours to degrade lignin, then dissolve pectinase and β-glucanase in acetate buffer with pH 4.3, and use acetate buffer for every 1g of pectinase or 1g of β-glucanase dissolved The amount of liquid is 0.6ml, and then added to the straw raw materials, then add xylanase, cellulase and amylase, use 0.3ml xylanase, 3ml cellulase, starch 2ml of enzyme, 0.7g of pectinase and 0.4g of β-glucanase, the pH of the mixture was adjusted to 5.0 with dilute sulfuric acid, and then the enzymolysis was carried out at 50°C for 50h.

Embodiment 3

[0015] Embodiment 3: the compound enzymatic hydrolysis method of straw class raw material, comprises the following steps: the straw class raw material is pulverized first, then adds water 15 times of the weight of straw class raw material to adjust concentration, adds concentrated sulfuric acid so that the mass concentration of sulfuric acid in the solution is 1.5% , hydrolyze for 1 hour to degrade lignin, then dissolve pectinase and β-glucanase in acetate buffer with pH 5.5, and use acetate buffer for every 1g of pectinase or 1g of β-glucanase dissolved The amount of solution is 1.5ml, and then added to straw raw materials, then add xylanase, cellulase and amylase, use 0.5ml xylanase, 4ml cellulase, starch for every 100 grams of straw raw materials 3ml of enzyme, 1g of pectinase and 0.5g of β-glucanase, the pH of the mixture was adjusted to 5.5 with sodium hydroxide, and then the enzymolysis was carried out at 55°C for 24h.

[0016] Adopt DNS method to carry out reducing suga...

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PUM

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Abstract

The invention discloses a compound enzymatic hydrolysis method for straw raw materials, which comprises: hydrolyzing the straw raw materials by diluted acid to decompose lignin; adding xylanase, cellulose, amylase, pectinase and beta-glucanase, and adjusting the pH value of the mixture to 4.3 to 5.5 by using an acidic matter or alkaline matter; and performing enzymatic hydrolysis at 40 to 55 DEG C for 24 to 72 hours. In the invention, a compound enzyme method is used to perform enzymatic hydrolysis on straw to convert the straw into reducing sugar, a proper mixing ratio of compound enzymes is selected to allow enzymes to interact to maximize the hydrolysis, the concentration of the reducing sugar generated by the enzymatic hydrolysis is high, the yield of sugar is high, the enzymatic hydrolysis conditions are mild, adverse influences on fermentation in a later period are avoided, the growth of a strain for fermentation in the later period is not inhibited, the ethanol yield during fermentation is high, the investment and cost are small, and thus, the method is suitable for large-scale use in industry.

Description

technical field [0001] The invention belongs to the technical field of ethanol production from straw raw materials, and relates to a compound enzymatic hydrolysis method of straw raw materials. Background technique [0002] The energy crisis has become an increasingly serious problem in today's society. Petrochemical energy such as oil and coal is increasingly depleted, and there is an urgent need to develop new energy substitutes. Under the background of serious crisis and serious environmental pollution caused by traditional fossil energy such as coal and oil, fuel ethanol has been favored by the government and society for its advantages of low pollution, high combustion value, renewable, and the use of technology in motor vehicles. Pay attention to. If straw-like fiber raw materials are used to produce fuel ethanol, both resources and society will have good benefits. However, since most of the raw materials for the production of fuel ethanol currently use starch-based s...

Claims

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Application Information

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IPC IPC(8): C12P7/10
CPCY02E50/16Y02E50/10
Inventor 宋安东张百良张玲玲陈红歌王风芹谢慧任天宝
Owner HENAN AGRICULTURAL UNIVERSITY
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