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Gene recombinant vaccine for preventing enterovirus 71 infection and preparation method thereof

A gene recombination and vaccine technology, applied in the direction of antiviral agents, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of undeveloped vaccines with commercial application value in medicine, and achieve safe, reliable and easy preparation methods Promote the application, the effect of ease of use

Inactive Publication Date: 2011-05-18
中国疾病预防控制中心病毒病预防控制所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011]Despite the development of EV71 and Cox.A16 vaccines, there are still no commercially viable vaccines

Method used

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  • Gene recombinant vaccine for preventing enterovirus 71 infection and preparation method thereof
  • Gene recombinant vaccine for preventing enterovirus 71 infection and preparation method thereof
  • Gene recombinant vaccine for preventing enterovirus 71 infection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The recombinant oral vaccine for preventing EV71 virus infection of the present invention is a recombinant oral vaccine of LTB-SP55-SP70 fusion gene, which uses Escherichia coli heat-labile enterotoxin B subunit LTB as an immune enhancing adjuvant and EV71 virus shell The two polypeptides SP55 and SP70 in the protein VP1 are antigens, and the codons are optimized according to the preference of Escherichia coli. After gene synthesis, the prokaryotic expression system of rLTB-SP55-SP70 is constructed by genetic engineering technology to obtain high-purity recombinant expression The EV71 virus genetically engineered vaccine of the product.

[0033] The present invention develops the selection of the genetic engineering vaccine to the antigen and its gene: all EV71 viruses have genes encoding SP55 and SP70, which have the characteristics of sequence conservation, high expression, surface distribution, large molecular weight and particle structure, and strong antigenicity. T...

Embodiment 2

[0127]

[0128]

[0129]

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PUM

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Abstract

The invention discloses a gene recombinant vaccine for preventing enterovirus 71 (EV71) infection and a preparation method thereof. The inflection comprises multiple diseases related with the nervous system such as hand-foot-and-mouth disease, paralytic diseases of sterile meningitis, cephalitis and poliomyelitis and the like. Escherichia coli labile enterotoxin B subunit (LTB) is used as an immunological enhancement adjuvant, two fragments of linear neutralizing epitope SP55 and SP70 in EV71 virus coat protein VP1 are used as antigens, prokaryotic expression plasmids of LTB-SP55-SP70 fusion genes are constructed by using gene engineering technology, the plasmids are expressed in escherichia coli, and a recombinant expression product is purified for preparing the EV71 virus gene engineering vaccine.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals and relates to a vaccine and a preparation method thereof. Especially related to gene recombination for the prevention of hand, foot and mouth disease, aseptic meningitis, encephalitis and polio-like paralytic diseases caused by enterovirus 71 (EV71 virus) infection, etc. Vaccines and methods for their preparation. technical background [0002] Hand, foot and mouth disease is caused by viruses of the family Picornaviridae and the genus Enterovirus, and has become an important infectious disease that endangers the health of infants and young children and public health safety. HFMD is mainly caused by Enterovirus 71 (EV71) and Coxsackievirus A16 (Cox.A16). The clinical symptoms caused by infection with EV71 and Cox.A16 were indistinguishable. Infection with EV71 is often complicated by severe neuroinflammation, including viral meningitis, encephalitis, and paralysis similar to polio, resulting i...

Claims

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Application Information

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IPC IPC(8): A61K39/125A61K39/395A61P31/14
CPCY02A50/30
Inventor 毕胜利陈斯勇伊瑶贾志远李庆
Owner 中国疾病预防控制中心病毒病预防控制所
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