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Apparatus and method for analysis by capillary electrophoretic method

A technology of capillary electrophoresis and analysis device, which is applied in separation methods, peptide preparation methods, material analysis by electromagnetic means, etc., can solve the problems of lack of measurement accuracy, high cost, and low HbA1c measurement accuracy, and achieves low operating cost and operation. easy effect

Active Publication Date: 2011-05-04
ARKRAY INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immunoassay and enzymatic method have the advantage of being able to handle a large number of analytes because they can be applied to automatic analysis devices, but they lack measurement accuracy and analysis takes about 10 minutes
Moreover, the accuracy of HbA1c determination in the separation principle of affinity chromatography is low, and the analysis takes 2 minutes
In addition, the HPLC method is widely used as a method for measuring hemoglobin (for example, Patent Document 1, etc.), but a large and expensive dedicated device is required, and it is difficult to reduce the size and cost of the device, and the analysis takes about 1 minute.

Method used

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  • Apparatus and method for analysis by capillary electrophoretic method
  • Apparatus and method for analysis by capillary electrophoretic method
  • Apparatus and method for analysis by capillary electrophoretic method

Examples

Experimental program
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Effect test

Embodiment approach 1

[0187] figure 1 The electrophoresis chip used in the capillary electrophoresis analysis device of this example is shown. figure 1 (A) is a plan view of the electrophoresis chip of this example, figure 1 (B) is from figure 1(A) Cross-sectional view viewed in the I-I direction. In this drawing, the size, ratio, and the like of each component are different from actual ones for easy understanding. As shown in the figure, the electrophoretic chip 2 of this example is composed of an upper substrate 3a laminated on a lower substrate 3b. The aforementioned upper substrate 3a is formed with three through holes. The bottoms of the three through-holes formed in the upper substrate 3a are sealed by the lower substrate 3b to form three liquid tanks 4a, 4b, and 4e. An I-shaped groove is formed on the lower substrate 3b. The upper portion of the I-shaped groove formed on the lower substrate 3b is sealed by the upper substrate 3a to form a capillary channel 5x for sample analysis. ...

Embodiment approach 2

[0202] image 3 The electrophoresis chip used in the capillary electrophoresis analysis device of this example is shown. In this figure, with figure 1 The same parts are attached with the same symbols. image 3 (A) is a plan view of the electrophoresis chip of this example, image 3 (B) is from image 3 The cross-sectional view of (A) viewed in the I-I direction, image 3 (C) is from image 3 (A) Cross-sectional view viewed in the II-II direction. As shown in the figure, the electrophoretic chip 2 of this example is composed of an upper substrate 3a laminated on a lower substrate 3b. The above-mentioned upper substrate 3a is formed with a plurality of (four in this example) through holes. Bottoms of the four through holes formed in the upper substrate 3a are sealed by the lower substrate 3b to form four liquid tanks 4a to 4d. A cross-shaped groove is formed on the lower substrate 3b. The upper portion of the cross-shaped groove formed on the lower substrate 3b is sea...

Embodiment approach 3

[0211] Figure 4 The electrophoresis chip used in the capillary electrophoresis analysis device of this example is shown. In this figure, with figure 1 and image 3 The same parts are attached with the same symbols. Figure 4 (A) is a plan view of the electrophoresis chip of this example, Figure 4 (B) is a perspective view of the electrophoresis chip of this example. As shown in the figure, the electrophoretic chip 200 of this example includes a laminate in which an upper substrate 3 a is laminated on a lower substrate 3 b, and a connector 70 . The connector 70 is disposed on one side of the laminated body. A wiring pattern (not shown) is formed on the lower substrate 3b. The aforementioned upper substrate 3a is formed with six through holes. The bottoms of the aforementioned six through holes are sealed by the aforementioned lower substrate 3b, thereby forming six liquid tanks. The aforementioned six liquid tanks are respectively the sample introduction port 41 , th...

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Abstract

Disclosed is an apparatus for analysis by a capillary electrophoretic method that has a wholly reduced apparatus size, can realize a simple operation and a low running cost, and can analyze a protein contained in a blood in a short time with a high accuracy. The apparatus for analysis by a capillary electrophoretic method is an analyzer for analyzing a blood protein by a capillary electrophoretic method. The analyzer comprises an electrophoresis chip, a voltage application means, a liquid feed means, and an absorbance measuring means. The electrophoresis chip comprises a substrate, a plurality of liquid tanks, and a capillary flow passage. The plurality of liquid tanks and the capillary flow passage are provided on the substrate. The plurality of liquid tanks are communicated with each other through the capillary flow passage. The capillary flow passage includes a capillary flow passage for a sample analysis, and the time taken for the analysis of the blood protein is not more than 35 seconds.

Description

technical field [0001] The present invention relates to an analysis device and analysis method for analyzing proteins contained in blood by capillary electrophoresis. Background technique [0002] Blood contains albumin, globulin (α1, α2, β, γ globulin), fibrinogen, hemoglobin and other proteins. The ratios, changes, etc. of these proteins are analyzed and utilized in the diagnosis of diseases. Among these proteins, albumin, globulin (α1, α2, β, and γ globulin), and fibrinogen are proteins contained in large amounts in blood. Furthermore, these protein ratios are important indicators in the diagnosis of, for example, liver cirrhosis, nephrotic syndrome, and collagen diseases, and are analyzed using cellulose acetate membrane electrophoresis or the like. In addition, hemoglobin (Hb) includes hemoglobin A (HbA), hemoglobin F (HbF), hemoglobin S (HbS), glycated hemoglobin, and the like. Among these hemoglobins, HbA and HbF are normal human hemoglobins. In addition, HbS is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
CPCB01D57/02C07K1/26G01N27/403G01N27/44721G01N27/44791G01N33/6893G01N33/721G01N33/726G01N2800/042
Inventor 杉山幸司东五十川行雄中山雄介田中喜秀
Owner ARKRAY INC
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