Organic fermenting bacterial preparation
A fermenting agent and organic technology, applied in the field of microbial fertilizers, can solve problems such as long fermentation time and environmental pollution, and achieve the effect of increasing yield and promoting growth
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Embodiment 1
[0020] ① Carry out amplified culture respectively:
[0021] After the yeast is activated on the slant, it is inoculated in the liquid medium and cultured at 35°C for 48 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween (80) 0.1 %, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.
[0022] After the thiolytic bacteria are activated on a slant, they are inoculated in a liquid medium and cultured at 28°C for 48 hours. The liquid medium formula: 1% glucose, 0.2% malt powder, 0.2% yeast extract, 0.5% sodium acetate, pH 7.0.
[0023] After the bacillus glialis is activated on the slant, it is inoculated in a liquid medium and cultured at 28°C for 48 hours. The liquid medium formula: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH6.5.
[0024] After the lactic acid bacteria are activated on the slant, they are inoculated in the liquid medium and cultivated at 30°C for...
Embodiment 2
[0027] ① Carry out amplified culture respectively:
[0028] After the yeast is activated on the slant, it is inoculated in the liquid medium and cultured at 35°C for 48 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween (80) 0.1 %, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.
[0029] After the thiolytic bacteria are activated on a slant, they are inoculated in a liquid medium and cultured at 28°C for 48 hours. The liquid medium formula: 1% glucose, 0.2% malt powder, 0.2% yeast extract, 0.5% sodium acetate, pH 7.0.
[0030] After the bacillus glialis is activated on the slant, it is inoculated in a liquid medium and cultured at 28°C for 48 hours. The liquid medium formula: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH6.5.
[0031] After the lactic acid bacteria are activated on the slant, they are inoculated in the liquid medium and cultivated at 30°C for...
Embodiment 3
[0034] ① Carry out amplified culture respectively:
[0035] After the yeast is activated on the slant, it is inoculated in the liquid medium and cultivated at 35°C for 36 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween (80) 0.1 %, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.
[0036] After the thiolytic bacteria are activated on a slant, they are inoculated in a liquid medium and cultured at 28°C for 36 hours. The liquid medium formula: 1% glucose, 0.2% malt powder, 0.2% yeast extract, 0.5% sodium acetate, pH 7.0.
[0037] After the bacillus glialis is activated on the slant, it is inoculated in a liquid medium and cultured at 28°C for 36 hours. The liquid medium formula: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH6.5.
[0038] After the lactic acid bacteria are activated on the slant, they are inoculated in the liquid medium and cultivated at 30°C f...
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