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Tremella fuciformis strain culture method

A cultivation method and technology of Tremella fungus strains, applied in botany equipment and methods, fertilizer mixtures, horticulture, etc., can solve the problems of easy degradation of strains, decreased resistance, aging of Tremella hyphae, etc., and achieve high yield and high resistance. Strong, stable growth effect

Inactive Publication Date: 2011-05-04
江寿根
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Problems solved by technology

However, the Tremella hyphae obtained by this method cannot be selected for the advantages and disadvantages of strains, and the cultured strains are easy to degrade, grow unstable, and the Tremella yield is not high, and there is no Tremella mycelium for selective propagation after long-term use. Aging is prone to occur, and resistance will also decline

Method used

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Embodiment Construction

[0016] In order to describe the technical content, structural features, achieved goals and effects of the present invention in detail, the following will be described in detail in conjunction with the embodiments and accompanying drawings.

[0017] Tremella fungus classification cultivation method of the present invention comprises the steps:

[0018] (1). Tremella leaves are put into a sterile liquid to clean, and then the spore liquid is separated and extracted; the sterile liquid can be a liquid obtained by clear water through high-temperature sterilization;

[0019] (2). The spore liquid extracted by separation is put into culture in the test tube culture medium (PDA test tube culture medium) that is formed by potato, glucose, agar and water, is cultivated at a suitable temperature (22 degrees Celsius), and after about 10 In about a day, the spores germinate into the size of sesame seeds;

[0020] The test tube culture medium can also be composed of sawdust and oatmeal. T...

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Abstract

The invention discloses a Tremella fuciformis strain culture method comprising the following steps of: soaking a Tremella fuciformis blade in sterile liquid, and then separating and extracting spore liquid; culturing the separated and extracted spore liquid in a culture medium; sorting out strong hyphae germinated by Tremella fuciformis spores from the culture medium and then putting the hyphae in a sterilized solid culture medium, and meanwhile, putting cultured Hypoxylon sp hyphae to the solid culture medium for performing pairing culture with and the Tremella fuciformis hyphae. In the Tremella fuciformis strain culture method, the strong hyphae germinated by the Tremella fuciformis spores are sorted out for culture, and then the pairing culture is carried out on the Tremella fuciformis hyphae and the strong Hypoxylon sp hyphae, thereby, the cultured Tremella fuciformis strain has strong resistance, stable growth and high yield and is not easy to degenerate.

Description

technical field [0001] The invention relates to a method for cultivating fungus strains, in particular to a method for cultivating tremella fungus strains. Background technique [0002] Along with the continuous development and growth of the edible fungus industry, the production of white fungus has become one of the main sources of income of most farmers in some areas. However, the life history of Tremella is relatively complex, and it is still quite difficult to obtain and preserve pure mycelium of Tremella. If only white fungus pure mycelium is inoculated on common culture medium (the medium that is made up of potato, glucose, agar) and cultivated, the mycelium grows very slowly, and easily transforms into arthrospores, and the mycelia tends to turn yellow and become gelatinous. change. Tremella mycelium does not have the ability to decompose lignocellulose, so it cannot grow and develop on lignocellulose alone. [0003] The cultivation of existing white fungus general...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 江寿根
Owner 江寿根
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