Bacillussubtilis for decomposing aflatoxin and active protein secreted by same

Abstract

The invention relates to a bacillussubtilis (Bacillussubtilis ANSB324 CGMCC No.3609) for decomposing aflatoxin, protein secreted by the same and an application thereof. The invention also provides a method for decomposing aflatoxin B1, G1 and M1 by using fermentation liquor of the strain or the protein secreted by the strain. By the method, after the bacillussubtilis and the aflatoxin B1 react for 48 hours, the decomposition rate of the B1 reaches 85%; after the bacillussubtilis and the aflatoxin G1 react for 72 hours, the decomposition rate of the G1 reaches about 66%; and after the bacillussubtilis and the aflatoxin M1 react for 72 hours, the decomposition rate of the M1 reaches about 68%. For the bacillussubtilis and the active protein secreted by the bacillussubtilis, the detoxifying activity is high, the specificity is strong, the action effect is mild, therefore, the bacillussubtilis provided by the invention is applicable to decomposing and removing the aflatoxin in feedstuff. Simultaneously, the bacillussubtilis provided by the invention has the advantages of strong bioactivity, good probiotics and stress resistance, and the like, and does not damage nutrient components inthe feedstuff.

Description

technical field [0001] The invention relates to bacillus subtilis, in particular to a bacillus subtilis used for degrading aflatoxin B1, G1 and M1 and the secreted active protein thereof. Background technique [0002] Mycotoxins are secondary metabolites produced during the growth of molds. Mycotoxin contamination has been a major threat to the global livestock and food industries. Mycotoxins are highly toxic, seriously threatening animal production performance and human health, and bringing huge economic losses to the feed industry and animal husbandry production every year. According to the Food and Agriculture Organization of the United Nations (FAO), it is estimated that 25% of the world's grain supply is contaminated with mycotoxins and cannot be eaten. At present, the mycotoxins that pose the greatest threat to animal husbandry include aflatoxins, trichothecenes, and zearalenone. Mycotoxins cause such serious harm to animal husbandry and humans, so since their disco...

AI Technical Summary

Problems solved by technology

The detoxification studies of some other bacteria and fungi have shown that the degradation efficiency of most bacteria is not high, and the degradation efficiency is affected by the action time, pH value of the solution, the number of bacteria, the concentration of toxins, and the concentration of metal ions. Irreversible degradation of aflatoxins in feed

[0004] So far, there is no report about the simultaneous degradation of aflatoxin B1, G1 and M1 by Bacillus subtilis and its extracellular enzymes

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