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Microbial biomass, feed product/ingredient and processes for production thereof

A technology of microbial biomass and microorganisms, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problem of expensive sources of microalgae

Inactive Publication Date: 2011-04-13
COMMONWEALTH SCI & IND RES ORG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In contrast, although indoor closed systems allow for the cultivation of single species of algae, they are too expensive as a source of microalgae for the aquaculture industry, making them more suitable for high-value applications such as pharmaceutical production

Method used

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  • Microbial biomass, feed product/ingredient and processes for production thereof
  • Microbial biomass, feed product/ingredient and processes for production thereof
  • Microbial biomass, feed product/ingredient and processes for production thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 (cultivation of microbial biomass)

[0060] To obtain an indication of optimal levels of nitrogen and readily available carbon sources in microbial biomass production, a first experiment was performed. The experiment consisted of 12 treatments, where each group consisted of two identical tanks. The treatments included 4 nitrogen (N) concentrations in 12 tanks (Table 1). The nitrogen source is urea fertilizer. Organic carbon in the form of tapioca starch was added to two identical tanks with each concentration of N for C:N ratios of 6:1, 12:1 and 18:1 (Table 1). The tank used to grow the microbial biomass was a circular fiberglass tank (2450L working volume) located in a horticultural tunnel house with minimal attenuation of natural light and maintained water temperature at 25-33°C.

[0061] The tanks were filled with filtered seawater (20 μm) and the experiment was started 5 weeks later. Additional microorganisms were added by adding 20 L of unfiltered se...

Embodiment 2

[0068] Example 2 (Cultivation of microbial biomass using lupine pods)

[0069] This example was performed to evaluate the use of lupine pods as a low-cost carbon source for microbial biomass cultivation and to compare the yields obtained using lupine pods and tapioca starch. The lupine pods are a by-product of processing lupine seeds to obtain lupine kernels. Samples of lupine pods were passed through a hammer mill to reduce their size and thus provide a greater surface area to volume ratio. This is expected to increase the rate of biotransformation of lupine pod material by the microbial community in the culture tank. The ground material appeared to contain two broad particle size fractions which were sieved with a 710 μm sieve to separate them. The two fractions provide two carbon sources. Analysis of the fractions revealed minor differences in similar composition (Table 3). Refined lupine fiber is included as an additional treatment, a product that is commercially pre...

Embodiment 3

[0083] The goal of this study was to investigate differences in the nutritional value of various microbial biomass cultures when added to feeds for black tiger shrimp - Penaeus monodon . The microbial biomass was produced as outlined in Examples 1 and 2 at Cleveland using CSIRO equipment in a 2500L tank. This study was conducted in the Clearwater Aquarium system.

[0084] The experiments involved using a basal diet and both containing 100 g kg -1 A 35-day feeding trial with a series of 15 diets of dry microbial biomass from different culture batches. The formulations of the basal diet and the three test diets are shown in Table 1 to illustrate the manner in which the diets were formulated.

[0085] During the preparation process, crude protein content and fat content (75g kg -1 DM) were maintained at the same level in all diets (420g kg -1 and 75g kg -1 DM). The same level (100 g kg -1 ), and adjust the amount of casein, vegetable oil mixture and wheat starch to balance...

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Abstract

This invention provides a process for producing a microbial biomass comprising: a) providing a mixed population of microorganisms comprising micro algae and bacteria; b) adding a carbon source to the mixed population of microorganisms; c) adding a nitrogen source to the mixed population of microorganisms; d) culturing the mixed population of microorganisms under conditions suitable for the growth of both the microalgae and bacteria to form a microbial biomass; and e) harvesting the microbial biomass. This invention also provides a microbial biomass, a feed product or ingredient, uses of microbial biomass and feed product or ingredient and a method of rearing an aquatic species using the microbial biomass and feed product or ingredient of this invention.

Description

technical field [0001] The present invention relates to a microbial biomass, feed product or component for aquatic species, a method for producing microbial biomass, a method for producing a feed product or component, using said microbial biomass and said Feed Products or Components Methods of farming aquatic species and uses of the microbial biomass and feed products or components of the invention. In particular, the present invention relates to microbial biomass, feed products or components, methods for producing microbial biomass and feed products or components, farming of aquatic species using said microbial biomass and said feed products or components Methods and uses of said microbial biomass and said feed products or components utilizing a mixed population of microorganisms comprising microalgae and bacteria. Background technique [0002] Microalgae are used in aquaculture as feed for molluscs, crustaceans and certain fish species, and for zooplankton used in the aqu...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/12A23K1/00
CPCC12N1/12A23K1/007A23K1/009C12P39/00A23K1/188C12N1/20A23K10/12A23K10/18A23K50/80Y02A40/818
Inventor D·M·史密斯N·P·普雷斯顿
Owner COMMONWEALTH SCI & IND RES ORG
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