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Detection method for flavonoids compounds in cotton rose general flavone

A flavonoid compound and detection method technology, which is applied in the field of detection of flavonoid compounds in total flavonoids of hibiscus hibiscus, can solve the problems of simultaneous qualitative and quantitative detection of flavonoid components and troublesome detection, and achieve easy mastery, high precision, and uniform variety Effect

Active Publication Date: 2011-04-13
陕西汉唐制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] It can be seen that it is cumbersome to use the above-mentioned method to detect several flavonoid components qualitatively and quantitatively at the same time; and in the same high performance liquid chromatography system, there is no report on the qualitative and quantitative detection of multiple flavonoid components at the same time

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  • Detection method for flavonoids compounds in cotton rose general flavone
  • Detection method for flavonoids compounds in cotton rose general flavone
  • Detection method for flavonoids compounds in cotton rose general flavone

Examples

Experimental program
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preparation example Construction

[0101] Preparation of the test solution:

[0102] Weigh 0.5g of the total flavonoids of Hibiscus hibiscus, add appropriate amount of distilled water after weighing accurately, and ultrasonically dissolve, the power is 200w, the frequency is 40kHz, and the time is 10min; then dilute to 50ml with water, add petroleum ether (60°C-90°C) to extract three times, 50ml each time, discard the petroleum ether solution, add ethyl acetate to the aqueous solution for extraction three times, 50ml each time, combine the ethyl acetate solution and evaporate to dryness under reduced pressure, dissolve the residue with methanol, dilute to 50ml, pass through 0.45μm micro Pore ​​filter membrane, promptly needs testing solution;

[0103] Preparation of reference solution:

[0104] Accurately weigh the appropriate amount of rutin, hyperin and quercitrin reference substances, add methanol to make mixed solutions containing 40.0 μg, 32.2 μg, and 28.0 μg per 1 mL, respectively;

[0105] The detectio...

Embodiment 1

[0151] Preparation of the test solution: Weigh about 0.5g of hibiscus total flavonoids extract, place it in a stoppered Erlenmeyer flask after weighing accurately, add appropriate amount of distilled water, ultrasonically dissolve, power 200w, frequency 40kHz, time 10min, dilute with water to 50ml , then add petroleum ether (boiling point 60°C-90°C) for extraction three times, 50ml each time, discard the petroleum ether solution, add ethyl acetate to the aqueous solution for extraction three times, 50ml each time, combine the ethyl acetate solution and evaporate to dryness under reduced pressure, Add methanol to dissolve the residue, dilute to 50ml, and pass through a 0.45μm microporous membrane to obtain the product.

[0152] Preparation of reference substance solution: Accurately weigh appropriate amount of rutin, hyperin, and quercitrin reference substances, add methanol to make mixed solutions containing 40.0 μg, 32.2 μg, and 28.0 μg per 1 mL, respectively, to obtain the so...

Embodiment 2

[0158] Preparation of the test solution: Weigh about 0.5g of hibiscus total flavonoids extract, place it in a stoppered Erlenmeyer flask after weighing accurately, add appropriate amount of distilled water, ultrasonically dissolve, power 200w, frequency 40kHz, time 10min, dilute with water to 50ml , then add petroleum ether (boiling point 60°C-90°C) for extraction three times, 50ml each time, discard the petroleum ether solution, add ethyl acetate to the aqueous solution for extraction three times, 50ml each time, combine the ethyl acetate solution and evaporate to dryness under reduced pressure, Add methanol to dissolve the residue, dilute to 50ml, and pass through a 0.45μm microporous membrane to obtain the product.

[0159]Preparation of reference substance solution: Accurately weigh appropriate amount of rutin, hyperin, and quercitrin reference substances, add methanol to make solutions containing 40.0 μg, 32.2 μg, and 28.0 μg per 1 mL, respectively.

[0160] A negative co...

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Abstract

The invention discloses a detection method for flavonoids compounds in cotton rose general flavones. In the method, a high performance liquid chromatograph and an ultraviolet detector are adopted; the determination is carried out according to the D high performance liquid chromatography of the appendix VI of volume I of Chinese pharmacopoeia, 2005 edition; octadecylsilane chemically bonded silica is used as the filler: XBridge Shield RP18, 4.6mm*250mm, and 5mu m; a specific mobile phase is adopted; the detection wavelength is 370nm or 254nm; the sample size is 20mu L; the theoretical plate number calculated according to quercitrin is not less than 3000; and the prepared test article solution and reference substance solution are injected under the chromatograph detection condition and are detected by a high performance liquid chromatograph. The method can work out the content of the quercitrin in the general flavones according to the peak area of the quercitrin and qualitatively detects out rutin and hyperin according to the appearance time of each peak of the test article solution.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and relates to a method for detecting flavonoids in hibiscus hibiscus total flavonoids. Background technique [0002] Hibiscus is a Malvaceae plant, also known as frost-resistant flower, hibiscus wood, magnolia, big-leaf hibiscus, lower row cup, ground hibiscus, flower tree, dry hibiscus, nine-headed flower, etc., and it is cultivated in my country. Medicinal leaves, flowers, root bark, harvested in summer and autumn. Use fresh or dry for later use. The whole plant has the effect of clearing heat and detoxifying, cooling blood and detumescence. It is used for the initial onset of carbuncle, boils, burns, bruises, red eyes and swelling, lung abscess, cough, dysentery, leucorrhea of ​​women, pyelonephritis embolism. [0003] The main chemical components in Hibiscus hibiscus are flavonoids and sterols, and the isolated flavonoids include quercetin, kaempferol, rutin...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/78A61K36/185
Inventor 邓亚利王菊孙平川郭颖娟
Owner 陕西汉唐制药有限公司
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