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Method for purifying effective ingredients of cicer ariethnum L. bean sprouts

The technology of an active ingredient and a purification method is applied in the field of purification of the active ingredient of chickpea sprouts, and can solve the problems of unsuitability for industrialized production, complicated operation and high cost

Active Publication Date: 2011-04-06
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Silica gel column, dextran gel column and other methods can purify saponins and isoflavones with high purity, but the operation is complicated, the cost is high, and it is not suitable for industrial production

Method used

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  • Method for purifying effective ingredients of cicer ariethnum L. bean sprouts
  • Method for purifying effective ingredients of cicer ariethnum L. bean sprouts
  • Method for purifying effective ingredients of cicer ariethnum L. bean sprouts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] a. Select high-quality, plump, neat and glossy chickpeas, soak them, add 0.02mg / L gibberellin dissolved in 5mL ethanol, 100mg / L (NH 4 ) 2 SO 4 and a mixture of 0.02mg / L 2,4-dichlorophenoxyacetic acid dissolved in 5mL of ethanol first, then add the selected chickpeas, the water temperature is 25°C, and the water consumption is twice the weight of the chickpeas, and then Disinfection treatment, using a fully automatic germination machine to germinate, the temperature is 27 ° C, pouring water every 4 hours, germinating after 6-7 days, picking bean sprouts, and drying;

[0056] b. Grind the dried bean sprouts in step a, pass through a 80-mesh sieve, take 500g of bean sprouts powder with 30% ethanol at a concentration of 5 times the mass-volume ratio, heat and reflux for extraction for 1 hour, extract once, collect the extract, and recover ethanol under reduced pressure ;

[0057] c, absorb the extract of step b with polyamide, the mass ratio of chickpea bean sprouts powd...

Embodiment 2

[0060] a. Select high-quality, plump, neat and glossy chickpeas, soak them, add 0.10mg / L gibberellin dissolved in 5mL ethanol first, 150mg / L (NH 4 ) 2 SO 4 and a mixture of 0.10mg / L 2,4-dichlorophenoxyacetic acid dissolved in 5mL of ethanol first, then add the selected chickpeas, the water temperature is 28°C, and the water consumption is twice the weight of the chickpeas, and then Disinfection treatment, using a fully automatic germination machine to germinate, the temperature is 27 ° C, pouring water every 4 hours, germinating after 6-7 days, picking bean sprouts, and drying;

[0061] b. Grind the dried bean sprouts in step a, pass through a 90-mesh sieve, take 500g of bean sprouts powder with a mass-volume ratio of 8 times the concentration of 45% ethanol, heat and reflux for extraction for 2 hours, extract twice, collect the extract, and recover ethanol under reduced pressure ;

[0062] c, absorb the extract of step b with polyamide, the ratio of chickpea bean sprouts p...

Embodiment 3

[0065] a. Select high-quality, plump, neat and glossy chickpeas, soak them, add 0.50mg / L gibberellin dissolved in 5mL ethanol first, 200mg / L (NH 4 ) 2 SO 4 and a mixture of 0.50mg / L 2,4-dichlorophenoxyacetic acid dissolved in 5mL of ethanol first, then add the selected chickpeas, the water temperature is 30°C, and the water consumption is twice the weight of the chickpeas, and then Disinfection treatment, using a fully automatic germination machine to germinate, the temperature is 27 ° C, pouring water every 4 hours, germinating after 6-7 days, picking bean sprouts, and drying;

[0066] b. Grind the dried bean sprouts in step a, pass through a 100-mesh sieve, take 500g of bean sprouts powder with a mass-volume ratio of 15 times the concentration of 75% ethanol, heat and reflux for extraction for 3 hours, extract 4 times, collect the extract, and recover ethanol under reduced pressure ;

[0067] c, absorb the extract of step b with polyamide, the ratio of chickpea bean sprou...

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Abstract

The invention relates to a method for purifying effective ingredients of cicer ariethnum L. bean sprouts. The method comprises the following steps of: selecting superior cicer ariethnum L.; germinating; picking the bean sprouts; drying; crushing; adding alcohol; heating for refluxing and extracting; collecting the extracting solution; recycling the alcohol under reduced pressure to obtain the extracting solution; then purifying with polyamide which is a macroporous resin; eluting with alcohol solution; concentrating under reduced pressure; and drying in vacuum to obtain the effective ingredients of the cicer ariethnum L. bean sprouts, such as total saponins and total isoflavones. The effective ingredients of the cicer ariethnum L. bean sprouts such as total saponins and total isoflavones proved by an in vitro sugar reducing experiment have inhibition effect to protein tyrosine phosphatase 1B and have good activity. The content of the effective ingredients of the cicer ariethnum L. bean sprouts, such as total saponins and total isoflavones is up to 52%, which has good repeatability. The resin can be reused.

Description

Technical field: [0001] The invention relates to a method for purifying active ingredients of chickpea bean sprouts, in particular to a method for purifying total saponins and total isoflavones of chickpea bean sprouts. Background technique: [0002] Chickpea (Cicer arietinum L.) originated in West Asia and the Mediterranean coast, and the global planting area has reached 150 million mu. It is currently the second largest edible bean crop in the world. With a growth history of 10 years, it is a commonly used medicinal material in Uyghur medicine. It has been recorded in the Uyghur medicine sub-volume of "Drug Standards" and "Uyghur Medicine Chronicles" of the Ministry of Health of the People's Republic of China. For thin body, low libido, loss of appetite, skin itching and diabetes and other diseases. [0003] Chickpea sprouts are rich in saponins, mainly triterpenoid soybean saponins. We determined that chickpea sprouts contain about 5% saponins. Saponins have a variety ...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P39/00A61P15/00A61P1/14A61P17/04A61P3/10A61K131/00
Inventor 阿吉艾克拜尔·艾萨程珍阿布力米提·伊力吕俏莹高彦华窦君
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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