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Method of changing stomatal aperture and density of plant

A plant and stomatal technology, applied in the field of agricultural science, to achieve the effect of improving plant quality and adaptability

Inactive Publication Date: 2011-03-30
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no relevant reports on whether the red light receptor phyB regulates the stomatal physiological state (such as stomatal opening and stomatal density)

Method used

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  • Method of changing stomatal aperture and density of plant
  • Method of changing stomatal aperture and density of plant
  • Method of changing stomatal aperture and density of plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1: Construction of cry1cry2phyB triple mutant

[0104] Dicotyledonous seeds are chemically or physically mutagenized to create mutant libraries (see literature listed above for cry1, cry2 and phyB genes).

[0105] Seedlings of the M2 generation were screened for high hypocotyl height under strong blue light, weak blue light, red light and far red light. The mutants with increased hypocotyls obtained under weak blue light were transplanted into the soil, and the mutants with delayed flowering time were selected. The mutants with increased hypocotyls obtained under red light were transplanted into the soil, and the mutants with early flowering time were selected.

[0106] The CRY1, CRY2, PHYA, and PHYB sites of the above mutants were respectively amplified by PCR, and the mutation sites were determined by sequencing to obtain single mutants. After the determined cry1, cry2, phyA and phyB mutants are obtained, the cry1 and cry2 mutants can be crossed first to o...

Embodiment 2

[0107] Example 2: Construction of overexpressed CRY1, CRY2 and phyB transgenic plants

[0108] The full-length cDNAs of CRY1, CRY2 and PHYB (respectively SEQ IDNOs: 1, 3 and 5) were respectively obtained by PCR method, and inserted into the downstream of the 35S promoter of the pKY71 vector or PHB vector, respectively, to obtain the overexpression of CRY1, CRY2 and phyB respectively. Plant expression vector. These vectors are then used to transform plants respectively to obtain transgenic plants. under moderate intensity blue light (20 μmol·m -2 ·s -1 ), weak blue light (1-5μmol·m -2 ·s -1 ) and red light conditions to screen seedlings with significantly shortened hypocotyls, and the obtained transgenic plants were transgenic plants overexpressing CRY1, CRY2 and phyB, respectively.

Embodiment 3

[0109] Embodiment 3: the research of pore density and opening degree

[0110] Light source and instrument

[0111]The light source used when measuring the porosity coefficient is an LED screen, the wavelength of blue light is 469 nanometers, the wavelength of red light is 680 nanometers, and the wavelength of far-red light is 730 nanometers. The temperature under continuous light conditions was 22°C. The instruments used for light intensity measurement are Li250 light quantum measuring instrument and ILT1400-A photometer (ILT, MA, USA) of Li-Cor Company in the United States.

[0112] Water loss test

[0113] Take isolated rosette leaves of WT (wild type), phyB, cry1cry2, and cry1cry2phyB Arabidopsis plants that have been cultured for 21 days, and place them in a continuous 140 μmol m -2 ·s -1 Under the condition of incandescent fluorescent lamp irradiation, measure its water loss, expressed as the weight percent of initial fresh leaves (as described by Leung, J., Mer...

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Abstract

The invention provides a method of changing stomatal aperture and density of a plant. The method comprises adjusting a PHYB gene or a protein or peptide coded thereby, or adjusting a CRY1, CRY2 and / or PHYA gene or a combination of the protein or peptide coded thereby. The invention relates to a use of the PHYB gene or the protein or peptide coded thereby in adjusting the physiological states of the plant and the stomata, and a use thereof in adjusting environmental adaptability phenotype or behavior associated with the physiological states of the plant and the stomata. The method of the invention can be used for adjusting leaf water loss, transpiration rate and photosynthetic efficiency, improves plant drought resistance and water resistance or anti-waterlogging hydrophile or prolongs fresh-keeping time, and has certain economical significance.

Description

technical field [0001] The present invention relates to the field of agricultural sciences. Specifically, the present invention relates to a method for enhancing the ability of plants to adapt to the environment by changing the stomatal density and opening of plants. Background technique [0002] There are a variety of climatic conditions on the earth, including complex environments such as water shortage and rich water resources. It is of great significance to cultivate suitable crops and economic crop varieties in different environments, which is conducive to improving the environment and energy. usage efficiency. A detailed understanding of the mechanisms that regulate stomatal growth, development and motility is necessary to develop resilient crops. [0003] The stomata of higher plants are mainly distributed in the leaf epidermis, surrounded by two guard cells, which are the channels that plants must pass through to absorb carbon dioxide and water into and out of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H1/06A01H1/02
Inventor 杨洪全康春颖王芳芳
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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