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High-activity high-tolerance formaldehyde degrading bacteria and application thereof

A formaldehyde-degrading bacteria and high-activity technology, which is applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems that affect practical application, formaldehyde tolerance concentration and degradation activity are not high enough, and achieve application promising effect

Active Publication Date: 2011-03-30
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the formaldehyde-degrading bacteria reported at home and abroad have not high enough formaldehyde tolerance concentration and degradation activity, which affects their practical application.

Method used

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  • High-activity high-tolerance formaldehyde degrading bacteria and application thereof
  • High-activity high-tolerance formaldehyde degrading bacteria and application thereof
  • High-activity high-tolerance formaldehyde degrading bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1 Materials and methods

[0051] 1.1 Materials

[0052] 1.1.1 Source of bacteria

[0053] A strain that can use formaldehyde as the sole carbon source was isolated from the soil of the waste reagent disposal site of Zhejiang University of Technology.

[0054] 1.1.2 Medium and reagents

[0055] Basic medium composition: KH 2 PO 4 0.7g / L, K 2 HPO 4 0.85g / L, (NH 4 ) 2 SO 4 1.2g / L, MgSO 4 ·7H 2 O 0.1g / L, CaCl 2 0.01g / L, FeSO 4 ·7H 2 O 0.001g / L, trace element mother solution 0.1mL, solvent is water, pH 7.0, sterilized at 115°C for 30min.

[0056] Trace element mother liquor composition: H 3 BO 3 6g / L, CoCl 2 ·6H 2 O 4g / L, ZnSO 4 ·7H 2 O 2g / L, MnCl 2 4H 2 O 0.6g / L, Na 2 MoO 4 ·7H 2 O 0.6g / L, NiCl 2 ·6H 2 O 0.4g / L, CuCl 2 2H 2 O 0.2g / L, the solvent is water.

[0057] Acetylacetone solution: 50g ammonium acetate, 6mL glacial acetic acid and 0.5mL acetylacetone reagent were dissolved in 100mL water. This solution is stable for at least one mont...

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Abstract

The invention provides high-activity high-tolerance formaldehyde degrading bacteria and an application thereof in micro-biological degradation of formaldehyde. In the invention, Pseudomonas putida xyz-zjut is conserved at China Center for Type Culture Collection; the address is Wuhan University, Luojia Mountain, Wuhan City, Hubei Province, 430072; the conservation date is May 23, 2010; and the conservation number is CCTCC No: M 2010125. The invention has the beneficial effects that the high-activity high-tolerance formaldehyde degrading bacteria and the application thereof provide a basis for biological control and treatment of formaldehyde and have wide application prospect.

Description

(1) Technical field [0001] The invention relates to a high-activity and high-tolerance formaldehyde-degrading bacterium and its application. (2) Background technology [0002] In recent years, due to the extensive use of various decoration materials, coatings and adhesives, the concentration of various harmful substances in the indoor air has continued to increase. Among them, many substances are highly toxic or carcinogenic, and formaldehyde is one of them. Formaldehyde is a native toxin, which is prone to addition, oxidation, reduction, and polymerization reactions, and has good water solubility. Formaldehyde is teratogenic and carcinogenic, ranking second on the list of priority control of toxic chemicals in my country. Therefore, it is urgent to find countermeasures for the prevention and control of formaldehyde pollution in indoor air, which is of great significance to human health. [0003] Biological control, especially the method of degrading microorganisms, is on...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B01D53/84B01D53/72A62D3/02C12R1/40C02F101/36A62D101/28
CPCY02A50/20
Inventor 钟卫鸿金晶邱乐泉吴石金钟莉徐云郑重陈建孟
Owner ZHEJIANG UNIV OF TECH
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