Method and system for implementing typing based on polymerase chain reaction sequencing

Abstract

The invention discloses a method and system for polymerase chain reaction sequencing-based typing (PCR-SBT). The method comprises the following steps: a heterozygote site and a base sequence to be typed are interpreted through a computer program according to a sequencing result; the base sequence to be typed is compared to a typing database of a corresponding site, an alignment position relation between the base sequence to be typed and reference sequences in the typing database is recognized; an allelic genetype in the typing database is searched, a penalty score of the allelic genetype in the typing database is obtained according to a sequencing strategy; and a candidate type group set is obtained according to the penalty score of the allelic gene type in the typing database. In the SBT method and system provided by the invention, automatic recognition of candidate genetypes can be achieved through a computer and other devices, thereby improving typing efficiency; and the convenience is provided for confirmation and modification of typing of typing personnel through imaging interface display and other technical means, thus typing accuracy and typing efficiency are provided.

Description

technical field [0001] The present invention relates to allele typing technology, in particular to a polymerase chain reaction product sequencing-based typing (Polymerase Chain Reaction Sequencing-based Typing, PCR-SBT) realization method and system. Background technique [0002] HLA (Human Leucocyte Antigen, Human Leucocyte Antigen) is one of the most polymorphic gene systems found so far, and it is the main gene system that regulates the specific immune response of the human body and determines individual differences in disease susceptibility. Transplant rejection is closely related. [0003] The current international standard HLA typing techniques are PCR-SSP (Polymerase Chain Reaction Sequence-Specific Primers, sequence-specific primer polymerase chain reaction), PCR-SSO (Polymerase Chain Reaction sequence-Specific Oligonucleotide Probe Hybridization, polymerase chain reaction oligonucleotide Nucleotide probe hybridization) and PCR-SBT (Polymerase Chain Reaction Sequenc...

AI Technical Summary

Problems solved by technology

[0008] However, the PCR-SBT method requires high equipment, consumes a lot of time and cost, and the existing technology is slow and inefficient when screening and retrieving large-scale candidate types.

In addition, in the manual-assisted typing stage, when the typing personnel check the electropherogram, the sequence cannot be synchronized with the electropherogram, and it cannot be viewed by adjusting the electropherogram

At the same time, the backup and recovery of data results cannot be realized, which may easily cause data loss

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