Fluorescence quantitative polymerase chain reaction (PCR) kit for quickly detecting herpes simplex viruses 1

Abstract

The invention discloses a fluorescence quantitative polymerase chain reaction (PCR) kit for quickly detecting herpes simplex viruses (HSV) 1, relates to technology of detecting a pathogene gene causing a plurality of diseases in people and is applied to the qualitative and quantative detection of the herpes simplex viruses 1. The kit consist of DNA extraction liquid, fluorescence quantitative PCR reaction liquid, PMD18-T-HSV1 serving as a standard positive template of the HSV1 and a negative quality control standard product, wherein the fluorescence quantitative PCR reaction liquid contains a special primer pair of the herpes simplex viruses 1 and a fluorescent probe. The kit is accurate in quantification, high in detection speed and high in specificity and sensitivity, can identify and detect the herpes simplex viruses 1 and has simple using steps and high repeatability. The kit can perform qualitative and quantitative detection on the herpes simplex viruses 1 quickly and can replace the traditional culture method which is continuously used.

Description

technical field [0001] The invention relates to a gene detection technology for pathogens causing various human diseases, in particular to a rapid detection of herpes simplex virus type I fluorescent quantitative PCR kit, which is suitable for qualitative and quantitative detection of herpes simplex virus type I. Background technique [0002] Herpes simplex virus (HSV) is a common pathogen that seriously endangers human health and causes various human diseases. HSV has two subtypes, HSV1 and HSV2. The nucleotide homology of the two subtypes reaches 50%, but the infection mode and clinical manifestations are different. [0003] HSV1 is mainly infected through contact with oral secretions, and the infection rate in the population is as high as 50-90%. The most common infection sites are the mouth and lips, which can cause primary infection, latent infection and recurrence. HSV1 primary infection often causes oropharyngeal herpes, herpetic keratoconjunctivitis, encephalitis, a...

AI Technical Summary

Problems solved by technology

[0006] The traditional culture method and serological method in clinical detection have the disadvantages of low sensitivity, poor specificity, false positive, time-consuming and labor-intensive; although the conventional PCR method has the advantages of simplicity, rapidity and sensitivity, it has the disadvantages of inaccurate quantification and PCR post-processing. False positives caused by pollution; therefore, it is necessary to develop an accurate, sensitive, rapid and pollution-free clinical testing method