Complex enzyme formula for improving extraction rate of sodium alginate and application

A sodium alginate and extraction rate technology, applied in the direction of enzymes, lyases, hydrolases, etc., can solve the problems of difficult to crack cell walls and achieve the effect of easy release

Inactive Publication Date: 2012-05-23
SHANDONG GANGYUAN MARINE BIOENG CO LTD
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extracting sodium alginate by enzymatic hydrolysis extraction is not mature enough at present, only a small amount of literature reports, such as "the research of cellulase extracting sodium alginate" such as Ma Chenghao (Guangzhou Food Industry Science and Technology, 2004, 20 (4): 12 ) article introduces single pretreatment with cellulase, and then extracts sodium alginate with traditional methods, which improves the extraction rate by 21.13% compared with single traditional process; , 35(12): 3661) established the traditional process steps such as cellulase action and then alkali digestion to produce sodium alginate, and its yield was improved to a certain extent.
The above method only uses a single cellulase for hydrolysis, but there are cellulase, hemicellulase, pectin, lignin and other components that make up the plant cell wall. The links between them are either mosaic or cohesive, so use It is difficult for a single enzyme to completely crack the cell wall of the plant and cannot release the contents completely

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Complex enzyme formula for improving extraction rate of sodium alginate and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] 2. The preparation method of the present invention is as follows: the raw material enzymes are pretreated respectively, and then put into an automatic mixer for compounding according to the proportion, and then the enzyme activity is measured and packaged quantitatively to become a product.

[0019] 3. The use method of the present invention: remove impurities from kelp or sargassum, add compound enzyme after crushing, stir evenly, add in a ratio of 5 / 1000 to 5 / 10,000, enzymatically hydrolyze 1- 6h, after the end of enzymatic hydrolysis, extract according to the normal process.

[0020] 4, process condition of the present invention:

[0021] Action temperature: 15-60°C (or room temperature) pH value: 4.0-9.0

[0022] Action time: 1-6h Addition amount: 500g-5000g tons of material

[0023] 5. Application effect:

[0024] Application of the present invention utilizes the biological method to essentially solve the fatal problems of low extraction yield and low viscosity ...

Embodiment 1

[0030] Embodiment 1: the specific formula of the present invention is as follows (content is the weight percentage of composition): protease 50%, cellulase 30%, pectinase 10%, xylanase 10%.

[0031] The preparation method is as follows: the raw material enzymes are subjected to pretreatment respectively, filtered by a microfiltration membrane, then mixed in an automatic mixer in proportion, filtered through a fine filtration membrane, and quantitatively filled to become a product.

Embodiment 2

[0032] Embodiment 2: The specific formula of the present invention is as follows (the content is the composition of weight percentage): 30% of protease, 30% of cellulase, 30% of pectinase, 10% of xylanase. The preparation method is the same as Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a complex enzyme formula for improving the extraction rate of sodium alginate and application, and belongs to the technical field of enzymology. The complex enzyme consists of a plurality of biological enzymes, is suitable for the industrial production of extracting and separating the sodium alginate from kelp and sargasso, can improve the extraction rate and the viscosity of the sodium alginate in the kelp and the sargasso, and simultaneously reduces the dosage of the chemical reagents.

Description

technical field [0001] The invention relates to a compound enzyme formula capable of improving the extraction rate of sodium alginate in kelp and sargassum and its application. The formula is as follows: protease 30-50%, cellulase 10-30%, pectinase 10-30%, Xylanase 5-15%. technical background [0002] Sodium alginate is a polysaccharide carbohydrate extracted from brown algae kelp or sargassum, which is composed of 1,4-poly-β-D-mannuronic acid and α-L-zanuronic acid. A linear polymer, one of alginic acid derivatives. Its molecular formula is (C 6 h 7 o 6 Na) n , and the relative molecular weight is around 32,000 to 200,000. [0003] Sodium alginate is widely used in chemistry, biology, medicine, food and other fields due to its good biodegradability and biocompatibility. At present, the methods of extracting sodium alginate in the world can be divided into four kinds of processes, namely: acid coagulation acidification method, calcium coagulation acidification method,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N9/00C12N9/50C12N9/42C12N9/26C12N9/88C08B37/04
Inventor 赵岩张连学吴鹏蔡恩博郜玉钢
Owner SHANDONG GANGYUAN MARINE BIOENG CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap