Complex enzyme formula for improving extraction rate of sodium alginate and application

Abstract

The invention relates to a complex enzyme formula for improving the extraction rate of sodium alginate and application, and belongs to the technical field of enzymology. The complex enzyme consists of a plurality of biological enzymes, is suitable for the industrial production of extracting and separating the sodium alginate from kelp and sargasso, can improve the extraction rate and the viscosity of the sodium alginate in the kelp and the sargasso, and simultaneously reduces the dosage of the chemical reagents.

Description

technical field [0001] The invention relates to a compound enzyme formula capable of improving the extraction rate of sodium alginate in kelp and sargassum and its application. The formula is as follows: protease 30-50%, cellulase 10-30%, pectinase 10-30%, Xylanase 5-15%. technical background [0002] Sodium alginate is a polysaccharide carbohydrate extracted from brown algae kelp or sargassum, which is composed of 1,4-poly-β-D-mannuronic acid and α-L-zanuronic acid. A linear polymer, one of alginic acid derivatives. Its molecular formula is (C 6 h 7 o 6 Na) n , and the relative molecular weight is around 32,000 to 200,000. [0003] Sodium alginate is widely used in chemistry, biology, medicine, food and other fields due to its good biodegradability and biocompatibility. At present, the methods of extracting sodium alginate in the world can be divided into four kinds of processes, namely: acid coagulation acidification method, calcium coagulation acidification method,...

AI Technical Summary

Problems solved by technology

Extracting sodium alginate by enzymatic hydrolysis extraction is not mature enough at present, only a small amount of literature reports, such as "the research of cellulase extracting sodium alginate" such as Ma Chenghao (Guangzhou Food Industry Science and Technology, 2004, 20 (4): 12 ) article introduces single pretreatment with cellulase, and then extracts sodium alginate with traditional methods, which improves the extraction rate by 21.13% compared with single traditional process; , 35(12): 3661) established the traditional process steps such as cellulase action and then alkali digestion to produce sodium alginate, and its yield was improved to a certain extent.

The above method only uses a single cellulase for hydrolysis, but there are cellulase, hemicellulase, pectin, lignin and other components that make up the plant cell wall. The links between them are either mosaic or cohesive, so use It is difficult for a single enzyme to completely crack the cell wall of the plant and cannot release the contents completely

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