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Method for establishing enterovirus 71-type intraperitoneal inoculation infection BALB/c suckling mouse model

An enterovirus and a technology for establishing a method, applied in the direction of medical raw materials derived from viruses/bacteriophages, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve problems such as the lack of stable and mature animal models, and achieve broad application prospects Effect

Inactive Publication Date: 2011-02-23
INST OF BASIC MEDICINE OF SAMS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the pathogenic mechanism, clinical treatment and vaccine development of EV71 at home and abroad are hindered by the lack of stable and mature animal models

Method used

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  • Method for establishing enterovirus 71-type intraperitoneal inoculation infection BALB/c suckling mouse model
  • Method for establishing enterovirus 71-type intraperitoneal inoculation infection BALB/c suckling mouse model
  • Method for establishing enterovirus 71-type intraperitoneal inoculation infection BALB/c suckling mouse model

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Experimental program
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Effect test

Embodiment 1

[0015] Example 1 Establishment of a mouse model infected with enterovirus 71

[0016] Proceed as follows:

[0017] 1. Isolation and identification of EV71

[0018] The EV71 strain used in the present invention is isolated from the stool specimens of clinical severe hand-foot-mouth patients, and simultaneously uses human rhabdomyosarcoma cell line (RD), rhesus monkey kidney cell line (MA104) and African green monkey kidney cell line (Vero) Three kinds of cells were used to separate the viruses in the specimen. As a result, the bacteria on the three kinds of cells showed cytopathic changes, and the viruses were frozen and preserved after passage. Then extract the genomic RNA of the isolated EV71, reverse transcribe into cDNA, adopt the EV71 national standard primer to identify the isolated strain, the results show that the isolated strain has a very bright band at 226bp (such as figure 1 Shown), it can be confirmed that the strain isolated from the stool specimens of patients ...

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Abstract

The invention discloses a method for establishing an enterovirus 71-type (EV71) intraperitoneal inoculation infection BALB / c suckling mouse model. The EV71 infection mouse model is prepared by intraperitoneally injecting virus liquid into a BALB / c suckling mouse. The virus liquid is prepared by the following steps of: inoculating EV71 strains into MA104 cells, performing culture at the temperature of 37 DEG C by using 5 percent carbon dioxide (CO2), and observing the pathological change conditions of the cells every day, wherein on the next day of the EV71 inoculation, agglutination and shrinkage occur on 70 percent of the MA104 cells; and after freeze-thawing the whole bottle of cells, centrifugally taking supernatant to obtain the EV71 virus liquid. By the method, a stable enterovirus EV71 intraperitoneal inoculation infection BALB / c suckling mouse model is established successfully; and foundation is laid for scientific researchers to research EV71 etiology, an EV71 pathogenic mechanism, the screening of high-efficiency anti-virus medicines and the preparation of vaccines. The method has a wide application prospect.

Description

technical field [0001] The invention relates to a method for establishing a BALB / c suckling mouse model of enterovirus 71 intraperitoneal inoculation infection. Background technique [0002] Enterovirus 71 (EV71) belongs to the Enterovirus genus of the picornaviridae family and is one of the main pathogens causing hand, foot and mouth disease (HFMD) in infants. The main symptoms of EV71 infection are rashes or herpes on the hands, feet, mouth and other parts. Some children may develop neurological complications such as aseptic meningitis, brainstem encephalitis, neurogenic pulmonary edema, and polio-like paralysis. Respiratory tract infection and myocarditis etc., can be disabled, lethal. Since the outbreak of hand, foot and mouth disease in Fuyang, Anhui in 2008, the infection of EV71 has been intensified and has become a public health concern. Therapeutic drugs and vaccines. [0003] The lack of mature and stable animal models is one of the most important reasons for th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/76A61K35/765
Inventor 宋楠楠盖中涛李鹏岳盈盈李志会纪璇张颖孟红
Owner INST OF BASIC MEDICINE OF SAMS
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