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Method for extracting general flavones from bamboo leaves

A technology of total flavonoids and bamboo leaves, applied in the direction of organic chemistry, can solve the problems of high cost and large loss of active ingredients, and achieve the effect of low cost, simple process operation, cost safety and non-toxicity

Inactive Publication Date: 2011-02-16
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method adopts the method of ethanol precipitation to remove impurities, the cost is high, and the active ingredients such as flavonoids in the extract lose a lot

Method used

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  • Method for extracting general flavones from bamboo leaves

Examples

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example 1

[0021] 1. Extract total flavonoids from bamboo leaves

[0022] (1) Do the preparatory work earlier, that is, bamboo leaves are dried and pulverized into coarse powder; getting an equal amount of cellulase and pectinase and adding water to prepare a compound enzyme solution with a concentration of 0.1%; getting chitosan 0.8g, added to 80mL concentration of 1% acetic acid solution to make a chitosan colloid solution with a chitosan concentration of 1%, and set aside; take 4g of 101 fruit juice clarifier, add it to 80mL distilled water to make a concentration of 101 fruit juice clarifier It is 5% aqueous solution of 101 fruit juice clarifying agent, and it is reserved.

[0023] (2) Take 1000g of bamboo leaf powder, add 15 times of compound enzyme solution, then adjust the pH value of the solution to 5.0, then ultrasonically (50KHZ, 160W) at 50°C for 1.0h, then rapidly heat up to 90°C, keep Inactivate the enzyme for 5 minutes, filter, and concentrate the filtrate under reduced pr...

example 2

[0031] (1) Bamboo leaf is dried and ground into coarse powder; Get equal amount of cellulase and pectinase and add water and be prepared into the compound enzyme solution that concentration is 0.15%; Prepare the chitosan concentration by the same method of example 1 as 1% chitosan colloid solution and 101 fruit juice clarifying agent concentration are 5% 101 fruit juice clarifying agent aqueous solution, standby.

[0032] (2) Take 1000g of bamboo leaf powder, add 10 times of compound enzyme solution, then adjust the pH value of the solution to 4.5, then ultrasonically (50KHZ, 160W) at 40°C for 0.5h, then rapidly heat up to 90°C, keep Inactivate the enzyme for 5 minutes, filter, and concentrate the filtrate under reduced pressure to a relative density of 1.12 (60°C).

[0033] (3) At 60°C, first add 80 mL of chitosan colloid solution, stir well, then add 80 mL of 101 fruit juice clarifier aqueous solution, stir well, let stand overnight, centrifuge, and take the supernatant.

...

example 3

[0036] (1) Bamboo leaf is dried and ground into coarse powder; Get equal amount of cellulase and pectinase and add water and be prepared into the compound enzyme solution that concentration is 0.05%; Prepare the chitosan concentration by the same method of example 1 as 1% chitosan colloid solution and 101 fruit juice clarifying agent concentration are 5% 101 fruit juice clarifying agent aqueous solution, standby.

[0037] (2) Take 1000g of bamboo leaf powder, add 20 times of compound enzyme solution, then adjust the pH value of the solution to 6.0, then ultrasonically (50KHZ, 160W) at 60°C for 1.5h and then rapidly heat up to 85°C, keep Inactivate the enzyme for 5 minutes, filter, and concentrate the filtrate under reduced pressure to a relative density of 1.12 (60°C).

[0038] (3) At 60°C, first add 80 mL of chitosan colloid solution, stir well, then add 80 mL of 101 fruit juice clarifier aqueous solution, stir well, let stand overnight, centrifuge, and take the supernatant. ...

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Abstract

The invention relates to a method for extracting general flavones from bamboo leaves, which comprises the following steps of: drying and crushing bamboo leaves, and then adding 10-20 times of a complex enzyme solution with mass concentration of 0.05-0.15 percent; regulating the pH value of the solution to be 4.5-6; then ultrasonically treating for 0.5-1.5h at a temperature of 40-60 DEG C, then rapidly raising the temperature to 85-90 DEG C, keeping for 5min and inactivating enzyme, filtering, collecting filtrate, decompressing and concentrating until the relative density is 1.12, wherein the complex enzyme is a mixture of cellulase and pectinase which have mass proportion of 1:1; adding chitosan in a concentrated liquid according to the proportion of 80mL of chitosan colloidal solution to 1000mL of concentrated liquid, fully stirring, adding a 101 juice classifying agent water solution according to the proportion of 80mL of 101 juice clarifying agent water solution to 1000mL of concentrated liquid, fully stirring, standing over the night, centrifugally separating, taking supernate; and leading the supernate to pass through a D101 resin column, eluting with alcohol with volume concentration of 50 percent, concentrating eluant, decompressing and drying to obtain the general flavones of the bamboo leaves.

Description

technical field [0001] The present invention relates to heterocyclic compounds, in particular to heterocyclic compounds without hydrogenation of heterocyclic rings, especially to the preparation of flavonoids. Background technique [0002] my country is known as the "Kingdom of Bamboo", and bamboo leaves are rich in sources and widely distributed in regions. Bamboo leaves are the leaves of Phyllostachys heterocycla vat.pubescens of the genus Phyllostachys heterocycla vat. Bamboo leaves are rich in flavonoids, which have excellent anti-free radical, anti-oxidation, anti-aging, regulating blood lipid and blood cholesterol, regulating immunity, antibacterial, antiviral and other effects. It is safe and non-toxic, and has very broad application prospects in functional foods, medical and health products, and medicines. [0003] The flavonoids in bamboo leaves are mainly carbon glycoside flavonoids, and the four main bamboo leaf carbon glycoside flavonoids are Orientin, Homoorie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/04
Inventor 刘莉刘强
Owner SOUTHERN MEDICAL UNIVERSITY
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