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Simulation and detection method for content of microelements in medicine capable of being absorbed by human body

A technology of trace elements and detection methods, applied in the field of analog detection, can solve the problems of high cost, radiation hazards of whole-body tracer element counting method, large differences in physiological environment, etc.

Active Publication Date: 2011-02-09
MINNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage of solubility evaluation is that the absorbed or complexed trace elements cannot be measured, only the soluble non-heme trace elements can be studied; in the absorption evaluation, the chemical balance method is a comparative method for studying the absorption of trace elements in the human body. It is a good method, but it is cumbersome, time-consuming and expensive; the whole-body counting method has radiation hazards, and it is of little significance to measure the changes of trace elements in plasma after drug administration; human colon cancer cells (Caco- 2 cells) is an in vitro model used abroad in recent years to study the intestinal absorption of drugs, but the operation of the villus cell culture process is relatively difficult; the live experiments of white mice and other animals are quite different from the physiological environment of the human body, so it is used to verify The pharmacological effects of the drug, the accuracy of which is in question

Method used

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  • Simulation and detection method for content of microelements in medicine capable of being absorbed by human body
  • Simulation and detection method for content of microelements in medicine capable of being absorbed by human body
  • Simulation and detection method for content of microelements in medicine capable of being absorbed by human body

Examples

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Effect test

Embodiment 1

[0029]According to the traditional decoction method to obtain the decoction of traditional Chinese medicine, weigh 300g of safflower powder, put it into a 600mL beaker, add 400mL of ultrapure water each time, heat to boiling and keep it slightly boiling for about 1h, decoct three times, and combine three decoctions Boil the liquid, heat and concentrate, filter it with a 0.45 μm filter membrane and set the volume to 1000 mL to obtain 1000 mL of safflower decoction;

[0030] Take 250mL of safflower decoction and put it into a volumetric flask, then add 5mL of simulated saliva to it, shake at a constant temperature at 37°C for 5min; then add 150mL of simulated gastric juice, continue to shake at 37°C for 2h, and obtain 405mL in vitro gastric biomimetic digestion liquid;

[0031] Add 195mL duodenum simulated solution and 75mL simulated bile to the obtained 405mL safflower water decoction biomimetic digestion solution for exogastric cavity, continue shaking at 37°C for 7 hours, rem...

Embodiment 2

[0039] Obtain peach kernel water decoction according to the traditional decoction method, weigh 300g of peach kernel, put it into a 600mL beaker, add 400mL of ultrapure water each time, heat to boiling and keep slightly boiling for about 1h, decoct three times, and combine the decoction three times And heated and concentrated, filtered with a 0.45 μm membrane filter to 1000mL to obtain 1000mL peach kernel water decoction;

[0040] Take 250mL of peach kernel water decoction and put it into a volumetric flask, add 2.5mL of simulated saliva to it, shake at a constant temperature at 36°C for 6 minutes; then add 125mL of simulated gastric juice, continue to shake at 36°C for 4 hours, and obtain 377mL of in vitro gastric biomimetic digestive juice;

[0041] Add 178mL of duodenal simulated solution and 70mL of simulated bile to the 377mL in vitro bionic gastric digestion solution obtained above, continue shaking at 36°C for 10 hours, remove the digested solution and filter it with a 0...

Embodiment 3

[0049] Accurately weigh 2.50g of clematis powder into a 1000mL volumetric flask, add 7.5mL of saliva, shake in a water bath at a constant temperature of 38°C for 3 minutes, add 270mL of gastric juice, and continue shaking at 38°C for 1 hour to obtain gastric biomimetic digestion of clematis powder Add 332.5mL of duodenal juice and 140mL of bile to it, continue to shake at 38°C for 5h, filter with a 0.45μm filter membrane, and obtain 750mL of gastrointestinal full bionic digestion liquid of Clematis clematis powder, and store at 4°C stand-by.

[0050] Take 0.250 mg of egg yolk lecithin, dissolve it in 5.00 mL of chloroform to obtain a chloroform solution of egg yolk lecithin; transfer the above solution into a stoppered round-bottomed flask, and vacuum and rotate it on a rotary evaporator for 10 minutes to form Uniform multi-layer lipid film, and then fill it with nitrogen to prevent the multi-layer lipid film from being oxidized; add 25.00mL of the whole biomimetic digestion s...

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Abstract

The invention provides a simulation and detection method for the content of microelements in medicine capable of being absorbed by a human body, which mainly comprises the following steps: adding corresponding bionic digestive juices (simulated saliva, simulated gastric juice, simulated duodenal juice and simulated bile) in different digestion stages according to the components and contents of digestive juices secreted by different digestive organs in a period of time; then reasonably scheduling the digestion time in each stage according to the emptying time of different pharmaceutical dosageforms in the digestive organs of the human body to obtain full bionic digestive juice; then simulating the distributing and absorbing conditions of the full bionic digestive juice in the stomach and intestine of the human body by a single-layer liposome-water distribution system to obtain the single-layer liposome loaded with microelements; and then digesting by a digestion method, and measuring the contents of the microelements, thereby providing a new method for evaluating the effective dosage of a microelement complex in the medicine based on the drug effect, and simulating and detecting the contents of the microelements capable of being absorbed by the human body.

Description

technical field [0001] The invention relates to a simulated detection method, in particular to a simulated detection method for the content of trace elements in medicine which can be absorbed by the human body. Background technique [0002] Trace elements are closely related to human health. Trace elements in the human body can be divided into essential trace elements and non-essential trace elements according to different physiological functions. The trace elements necessary for the human body include iron, magnesium, zinc, calcium and copper, etc., in the human body It has a wide range of physiological functions, such as being a cofactor of various biologically active enzymes, participating in the synthesis of hormones and vitamins, and serving as an important electron transmitter. Trace elements can also play a role in drug absorption. The theory of drug coordination chemistry It is believed that trace element complexes are the real active ingredients of medicines in the ...

Claims

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Application Information

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IPC IPC(8): G01N33/15G01N1/28G01N1/44G01N21/31
Inventor 李顺兴郑凤英
Owner MINNAN NORMAL UNIV
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