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Composition for neutralizing botulinus toxin type-a, and human anti-botulinus toxin type-a antibody

A botulinum toxin and composition technology, applied in the direction of antibodies, bacteria, fungi, etc., can solve the problems of strengthening, not meeting expectations, and failing to obtain antibody clones

Active Publication Date: 2011-02-02
MEDICAL & BIOLOGICAL LAB CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for type A virus, antibody clones exhibiting sufficient neutralizing activity alone have not been successfully obtained
Therefore, one human antibody obtained as a result of phage display screening was mixed with one clone derived from XENO mice, and an antibody obtained by humanizing a mouse antibody obtained by immunizing the mouse was mixed (oligoclonalization). ), thus the neutralizing activity must be strengthened, and the desired purpose, that is, toxin neutralization caused by fully human neutralizing antibodies (Patent Documents 2, 3, Non-Patent Documents 4, 5, 6) has not yet been achieved

Method used

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  • Composition for neutralizing botulinus toxin type-a, and human anti-botulinus toxin type-a antibody
  • Composition for neutralizing botulinus toxin type-a, and human anti-botulinus toxin type-a antibody
  • Composition for neutralizing botulinus toxin type-a, and human anti-botulinus toxin type-a antibody

Examples

Experimental program
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Embodiment

[0273] 1. Preparation of Botulinum Toxoid Immune Antibody Library

[0274] 1-1. Inoculation of human volunteers with botulinum toxoid

[0275] Each type A, B, E, and F bacteria was cultured, and the toxin obtained by purifying the obtained toxin was used as a material to prepare a toxoid. In particular, type A is a neurotoxin produced by botulinum strain 97 (C. botulinum 97) or 62A strain (C. botulinum 62A), which is used as the current therapeutic toxin, according to the method carried out in type B neurotoxin ( Infect. Immun. 18:761-766, 1977) for purification. The purified various types of toxins are inactivated with formalin, and mixed with aluminum adjuvant to obtain precipitated ABEF type multiple mixed toxoids. Based on this polytoxoid-based immunization, 0.5 mL was subcutaneously injected three times at intervals of 3 to 4 weeks, and 0.5 mL was subcutaneously injected once about 12 months later. After 10 years and 20 years later, additional injections were given, an...

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Abstract

Disclosed is a means which is effective for botulism diseases and the prevention of the botulism diseases. Specifically disclosed are multiple human anti-botulinus toxin type-A antibodies having different epitopes from one another. Also specifically disclosed is a composition for neutralizing botulinus toxin type-A, which comprises a combination of two or more of the antibodies and which has a high neutralizing activity.

Description

technical field [0001] The present invention relates to a genetically recombinant antibody having binding activity to botulinum toxin type A and having a fully human variable region, a nucleic acid molecule encoding the antibody, a transformant producing the antibody, an antibody preparation method using the transformant, and using the Botulinum toxin type A neutralizing compositions for antibodies and the like. Background technique [0002] The toxins produced by Clostridium botulinum are classified into A-G types according to different serotypes. Types A, B, E, and F toxins have a major impact on human health. Among human botulism diseases, there are "food botulism (botulism food poisoning)", "infant botulism", "traumatic botulism" and "infectious botulism disease" 4 kinds. Type A toxin is associated with 26% of botulism food poisoning cases. Type A toxin is associated with 26% of botulism food poisoning cases. Types B and E followed, and type F was associated with a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/12C12N1/19C12P21/08C12N1/21C12N5/10C12N15/09C12N1/15A61K39/395
CPCC07K2317/565C07K2317/21C07K2317/92A61K2039/507A61K2039/505C07K16/1282C07K2317/55C07K2317/56C07K2316/96C07K2317/76A61P31/04
Inventor 东成见高桥元秀小崎俊司向本雅郁幸田知子黑泽仁黑泽良和
Owner MEDICAL & BIOLOGICAL LAB CO LTD
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