Echinococcosis granulosa gene engineering vaccine candidate P-29
A genetically engineered vaccine, the technology of Echinococcus granulosus, which is applied in the field of genetically engineered vaccines against hepatic hydatid disease, can solve the problems of toxic and side effects, drug resistance of chemotherapy patients, and intolerance of patients.
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[0009] Preparation of Echinococcus granulosus Vaccine Candidate Molecules
[0010] 1. Use bioinformatics technology to search for target gene fragments
[0011] First log in to the GenBank (National Institutes of Health) database (http: / / www.ncbi.nlm.nih.gov / GeneBank / ), enter the species name - Echinococcus granulosus (Echinococcus granulosus), and query through Entrez Nucleotide The published Echinococcus granulosus gene sequence was searched, and the P-29 gene sequence was selected as a candidate gene for genetic engineering vaccine.
[0012] 2. Synthetic primers
[0013] Primers were designed according to the base sequence of P-29, and the primer sequences are listed in Sequence Tables 2 to 3 respectively.
[0014] See sequence 2 for the primer at the 5' end of the P-29 gene; see sequence 3 for the primer at the 3' end of the P-29 gene.
[0015] 3. Extraction of total RNA from Echinococcus granulosus
[0016] Take 200 mg of protoscoleia fresh or frozen in liquid nitroge...
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