Gene chip for detecting mutation of 18 loci of susceptibility genes of type 2 diabetes

Abstract

The invention discloses a gene chip for detecting mutation of 18 loci of susceptibility genes of type 2 diabetes. The chip takes probes shown as SEQ ID No.1-36 which are fixed on a solid phase carrier as a detection system, and further comprises positive control, negative control and parallel control which form a quality control system package. The invention also discloses supporting primers for the detection of the gene chip. The primers can perform polymerase chain reaction (PCR) at the same annealing temperature, amplify and accomplish Cy3 marking at the same time, and perform hybridization reaction at one time to detect the 18 gene loci. The gene chip has the advantages of accurate detection result, fastness and high efficiency, can systematically screen the mutation of the 18 loci ofthe susceptibility genes of the type 2 diabetes which are reported at home and abroad at present, and is suitable for large-sample low-cost association studies on the susceptibility genes of the type2 diabetes.

Description

technical field [0001] The invention relates to the field of gene chip detection, in particular to a gene chip for rapidly detecting multiple site mutations of type 2 diabetes susceptibility genes, and the sites cover susceptibility genes clearly related to type 2 diabetes at present. Background technique [0002] Diabetes is a complex metabolic disease caused by defects in insulin secretion and / or insulin action. younger. Chronic complications caused by persistent hyperglycemia have become the main cause of renal failure, blindness and cardiovascular and cerebrovascular diseases, bringing a heavy burden to individual, social and national health care, and becoming a global social health and economic problem. Because T2DM has familial characteristics, and there are obvious differences in the incidence among different races, and the incidence and condition of identical twins and fraternal twins are also different, so genetic components play an important role in the pathogenes...

AI Technical Summary

Problems solved by technology

[0011] The traditional diabetes susceptibility gene detection methods mainly include: PCR-RFLP, AS-PCR and DNA sequencing methods, etc. These methods play an important role in the detection of gene mutations, but there are obvious deficiencies generally. The traditional diabetes susceptibility Gene detection methods mainly include: PCR-RFLP, AS-PCR and DNA sequencing methods, etc. These methods play an important role in the detection of gene mutations, but there are generally obvious deficiencies, such as limited detection of gene sites, long time-consuming, operation Complicated, etc., not suitable for large-scale and systematic testing, which brings difficulties to the clinical diagnosis of diabetes

[0012] The PCR-RFLP method is a classic biological method, but due to the limited restriction endonuclease recognition sites, it is difficult to detect multiple sites at the same time, and it takes a long time; although the AS-PCR method can accurately detect mutations, it requires The amplification conditions are very optimized, and the primers must be highly specific, otherwise false-limited or false-positive results are prone to occur; DNA sequencing is currently recognized as the gold standard for detecting new mutations, but it cannot be sequenced due to its special structural DNA sequence , and can only be detected when the heterogeneity level is > 25%, and the heterogeneity level in peripheral blood leukocytes of commonly used clinical samples usually does not meet this standard, so it is easy to miss detection

[0013] Currently commonly used SNaPshot and Sequenom technologies have high application costs and require the purchase of expensive special equipment

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