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High-density cultivation of functional bifidobacterium and preparation method of deep-frozen product thereof

A technology for high-density culture and bifidobacteria, which is applied in the field of bifidobacterium cultivation and the preparation of deep-frozen products, can solve the problems of high cost of manufacture and use of freeze-dried preparations, low content of viable bacteria, poor fermentation effect, etc., to achieve Low production cost, high activity, and load-reducing effect

Inactive Publication Date: 2011-01-19
HARBIN INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of low live bacteria content, large dosage, poor fermentation effect, and high cost of manufacture and use of the freeze-dried preparation in the existing production of bifidobacterium, and to provide functional bifidobacterium bifidobacteria. High-density culture of bacilli and preparation method of deep-frozen products thereof

Method used

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specific Embodiment approach 1

[0010] Specific embodiment one: The high-density cultivation of functional bifidobacteria in this embodiment is carried out according to the following steps: 1. Inoculate the functional bifidobacteria through the oxygen-resistant acclimation into the enrichment medium of the fermenter, at a temperature of 35 ~ Cultivate at 37°C and a pH value of 6.5 to 6.8 for 15 to 17 hours to obtain a fermentation broth; 2. When the fermentation broth is at 0 to 20°C, add a 10% sodium citrate solution or Sodium phosphate solution with a mass concentration of 10% elutes the protein in the fermentation broth, and then adds chitosan with a mass concentration of 0.2-2% to flocculate the bacteria; 3. The flocculated fermentation broth uses a pore size of 100-200nm , with an area of ​​0.5m 2 The ceramic membrane is used to carry out membrane concentration treatment at a transmembrane pressure of 110KPa until the concentration factor of the flocculated fermentation broth reaches 6, and the membrane...

specific Embodiment approach 2

[0014] Specific embodiment two: the difference between this embodiment and specific embodiment one is that a neutralizing agent is used in step one to adjust the pH value of the enrichment medium, and the neutralizing agent is 10% to 15% sodium hydroxide, mass concentration 10-15% ammonia water or 20% Na 2 CO 3 In the process of cultivating 15 to 17 hours in step 1, lactose needs to be added, and the amount of lactose to be added is 3 to 5 times the molar amount of alkaline substances in the neutralizer consumed, and the D301 resin produced during the adsorption culture process is used. sodium salt. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0015] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 or 2 is that in step 1, the fermentation broth is obtained by culturing for 17 hours at a temperature of 35° C. and a pH value of 6.5. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention discloses high-density cultivation of functional bifidobacterium and a preparation method of a deep-frozen product thereof, and relates to the cultivation of the bifidobacterium and the preparation method of the deep-frozen product thereof. The invention solves the problems that the traditional bifidobacterium has small viable bacteria content in the product, large dosage and bad fermentation effect in the production process and the freeze-dried preparation thereof has higher manufacturing cost and higher using cost. The cultivation comprises the steps of: (1) inoculating the aerotolerant naturalized functional bifidobacterium into an enrichment medium to obtain the fermentation liquor; (2) eluting and flocculating; and (3) carrying out membrane concentration to finish the cultivation. The preparation comprises the steps of: (1) mixing and pre-cooling a membrane concentration product and a cryoprotectant to obtain the mixture; and (2) pre-freezing the mixture, then crushing, packing and storing to obtain the finished product. The viable count of the bifidobacterium in the invention is 5.4*1010 CFU / g, therefore, the dosage is decreased, the fermentation effect is good; and the deep-frozen product has low production cost, simple process and high activity and achieves the purpose of directly using.

Description

technical field [0001] The invention relates to a method for preparing bifidobacterium culture and deep-frozen products thereof. Background technique [0002] Bifidobacteria are the most beneficial flora in the intestines. After fermentation in the human intestine, they can produce lactic acid and acetic acid, which can improve the utilization rate of calcium, phosphorus and iron, and promote the absorption of iron and vitamin D; Bifidobacteria ferment lactose to produce half Lactose is a component of cerebrosides in the brain nervous system, which is closely related to the rapid growth of the baby's brain after birth; Bifidobacteria can produce vitamins B1, B2, B6, B12 and alanine, valine, and aspartic acid Nutrients necessary for the human body such as acid and threonine have important nutritional effects that cannot be ignored for the human body; and they also have anti-aging, anti-allergic, anti-tumor, adjustment of intestinal function and improvement of nutrition. [0...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/01
Inventor 张兰威韩雪张英春冯镇杨林易华西杜明郭春峰
Owner HARBIN INST OF TECH
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