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PPAR (Peroxisome Proliferator-Activated Receptor) alpha gene and application thereof as goose fat traits genetic markers

A technology of α gene and traits, which is applied in the molecular biology technology and breeding field of goose, can solve the problems of no goose PPARα gene research report, few chickens, etc., and achieve great application value and economic benefits

Inactive Publication Date: 2010-12-22
曲湘勇 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, compared with chickens, there are few studies on the DNA genetic markers, genetic diversity and related functional genes of goose, and there is no research report on goose PPARα gene.

Method used

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  • PPAR (Peroxisome Proliferator-Activated Receptor) alpha gene and application thereof as goose fat traits genetic markers
  • PPAR (Peroxisome Proliferator-Activated Receptor) alpha gene and application thereof as goose fat traits genetic markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Goose related gene SNP screening

[0030] 200 commercial geese of the matching line of Dongting grass geese with the same age as healthy chicks were selected for feeding experiment. The high-abdominal fat type and low-abdominal fat type of these geese were identified, and the genomic DNA was extracted from the samples of the high-abdominal fat type and the low-abdominal fat type.

[0031] According to the mRNA sequence of chicken PPARα gene in GenBank, primers were designed with Primer 5.0 to cover the partial coding sequence of the gene. ID NO: 3, reverse), synthesized by Shanghai Yingwei Chuangjin Company. The above primers were used to amplify the extracted goose genomic DNA respectively.

[0032] PCR: 10μl Reaction system: 10×buffer (containing Mg 2+ ) 1 μl, upper and lower primers (20 μmol / L) each 0.15 μl, dNTPs (10 μmol / L) 0.2 μl, Taq enzyme 1U, DNA (100ng) 0.7 μl, ultrapure water to the required volume.

[0033] PCR amplification program: 30 PCR cyc...

Embodiment 2

[0040] Example 2 Distribution of Goose PPARα Gene Polymorphisms in Various Breeds

[0041] As shown in Table 1 below, it can be seen that the group sample belongs to an unbalanced group, which is basically consistent with the commercial geese that the group is a hybrid of two breeds. In the population tested in this study, the frequency of gene A was significantly lower than that of gene B; the frequency of homozygous genotypes AA and BB was low, while the frequency of heterozygous genotype was high.

[0042] Table 1 Gene frequency, genotype frequency table

[0043] genotype

Embodiment 3

[0044] Example 3 Verification of SNPs related to abdominal fat rate in production practice

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Abstract

The invention relates to a PPAR (Peroxisome Proliferator-Activated Receptor) alpha gene and application thereof as goose fat traits genetic markers. Genetic markers related to abdomen fat weight and abdomen fat ratio in the PPAR alpha gene are measured; the existence of polymorphism in the PPAR alpha gene in a nucleic acid sample obtained from a goose is measured, wherein the polymorphism is related to the abdomen fat weight and the abdomen fat ratio; and the fat traits selection breeding can be carried out on the goose containing the PPAR alpha gene in a genome DNA according to the single nucleotide polymorphism of the gene, and an advantageous genotype for reducing the abdomen fat weight and the abdomen fat ratio is obtained by the screening. The invention has extremely important application value and economic benefit.

Description

Technical field: [0001] The present invention relates to the field of molecular biology technology and breeding of goose, specifically relate to the existence of the hereditary marker of the feature related to determination and abdominal fat weight, abdominal fat rate, more specifically, it is to measure the polymorphism in the PPARα gene, namely with The existence of single nucleotide polymorphism (Single nucleotide polymorphism, abbreviated as SNP) of goose peroxisome proliferator-activated receptor (PPAR) gene related to abdominal fat weight and abdominal fat rate. Background technique: [0002] The development of modern poultry genetic breeding theory and application has greatly improved the growth rate and feed efficiency of broiler poultry, especially broiler chickens and ducks, and the production efficiency has gradually adapted to industrial production. But it is also easy to bring a large amount of deposition of carcass abdominal fat and subcutaneous fat, resulting ...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12Q1/68
Inventor 曲湘勇蒋隽何俊
Owner 曲湘勇
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