Three-layer electrostatic spinning ordered fiber nerve conduit and preparation and application thereof

Abstract

The invention relates to a three-layer electrostatic spinning ordered fiber nerve conduit and preparation and application thereof. The conduit comprises an inner layer, a middle layer and an outer layer, wherein the inner layer is a micron fibrous membrane, the middle layer is a polymer degradable reticulate conduit, and the outer layer is a submicron fibrous membrane with a three-dimensional reticulate structure. The preparation comprises the following steps of: (1) dissolving a PHBV in chloroform / acetone solution to prepare mixed solution; (2) performing electrostatic spinning on the mixed solution to prepare a fibrous membrane; (3) taking the fibrous membrane from a winding bobbin, and winding into an inner pipe; (4) weaving polymer degradable fibers into the reticulate conduit, and sleeving on the inner pipe; and (5) fixing a core rod sleeved with the inner pipe and the reticulate conduit on a high-speed winding and collecting device, and performing the electrostatic spinning on the mixed solution. The conduit has double functions of the nerve conduit and a tissue engineering support, and is favorable for the attachment of cells and the growth and the expansion of nerves along a fiber parallel direction; and the preparation method has the advantages of simpleness, no environmental pollution and suitability for industrialized production.

Description

technical field [0001] The invention belongs to the field of nerve guide and its preparation and application, in particular to a three-layer electrospinning ordered fiber nerve guide and its preparation and application. Background technique [0002] Since Hueter first proposed the comprehensive repair of peripheral nerve injuries by the adventitia in 1873, humans have made continuous efforts for the repair of peripheral nerves, and the repair methods have also been continuously improved. The main methods are: [0003] (1) The direct end-to-end suture method directly sutures the outer membranes of the two stumped nerves, and completes the repair by utilizing the regeneration of the nerves. This method is suitable for the repair of short-distance stumped nerves. This method cannot be implemented when the nerve stump distance is long. [0004] (2) Autologous nerve transplantation is currently the most commonly used clinical method and is considered the "gold standard" for long...

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Problems solved by technology

[Chen Nanliang et al., A double-layer braided nerve conduit and its preparation method: 200810200386.5[P].2009-02-18] invented the double-layer braided nerve conduit with an outer layer braided from polyglycolide-lactide The tube wall and the inner tube wall are composed of polyglycolide-lactide bonded parallel yarns, and the parallel yarns are bonded with a 3-4% (w / v) chitosan slurry coating, and the yarns are along the Conduits are arranged longitudinally, but they do not publish the denier of the yarn

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