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Method for preparing probiotics and ginkgo pollen oral solution

A technology for probiotics and oral liquid is applied in the field of preparation of probiotics ginkgo pollen oral liquid, which can solve problems such as dysbacteriosis, and achieve the effects of enhancing memory, improving immunity and having broad market prospects.

Inactive Publication Date: 2010-12-08
山东创新源农业技术开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, whether it is after surgery, or after recovery from acute or chronic diseases, patients have taken or injected a large amount of antibiotics in order to treat diseases or prevent infection, which has greatly changed the intestinal flora, and even some beneficial intestinal flora are also all inhibited or killed, resulting in an imbalance of flora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The method proceeds as follows:

[0024] a. fresh ginkgo pollen is placed in water according to fresh ginkgo pollen: water=1: 8 parts by weight, and then the ginkgo pollen is broken by a homogenizer with a rotating speed of 15000r / min for 10 minutes to obtain an aqueous solution of broken ginkgo pollen;

[0025] b. Introduce two probiotics, Lactobacillus rhamnosus and Lactobacillus acidophilus, into the MRS liquid seed medium, and culture them statically at 37°C for 10 hours to obtain two probiotic seed solutions;

[0026] c. get the broken ginkgo pollen aqueous solution of step a, mix according to the broken ginkgo pollen aqueous solution: sugar: sodium chloride: sodium glutamate=7:5:0.02:0.05 parts by weight, add 75 times the amount of deionized water Dissolve and sterilize at 120°C for 15 minutes to obtain a fermentation medium;

[0027] d. Take the two kinds of probiotic seed liquids in step b and combine them in a ratio of 1:1, then insert them into the fermentati...

Embodiment 2

[0029] The method proceeds as follows:

[0030] a. fresh ginkgo pollen is placed in water according to fresh ginkgo pollen: water=1: 10 parts by weight, then the ginkgo pollen is broken by a homogenizer at a speed of 10000r / min for 20 minutes to obtain an aqueous solution of broken ginkgo pollen;

[0031] b. Introduce two probiotics, Lactobacillus rhamnosus and Lactobacillus acidophilus, into the MRS liquid seed medium, and culture them statically at 30°C for 14 hours to obtain two probiotic seed solutions;

[0032] c. take the broken ginkgo pollen aqueous solution of step a, mix according to the broken ginkgo pollen aqueous solution: sugar: sodium chloride: sodium glutamate=10:3:0.05:0.02 parts by weight, add 80 times the amount of deionized water Dissolve and sterilize at 110°C for 30 minutes to obtain a fermentation medium;

[0033] d. Take the two kinds of probiotic seed liquids in step b and combine them in a ratio of 1:1, and then insert them into the fermentation mediu...

Embodiment 3

[0035] The method proceeds as follows:

[0036]a. Fresh ginkgo pollen is placed in water according to fresh ginkgo pollen: water=1: 9 parts by weight, and then the ginkgo pollen is broken by a homogenizer at a speed of 12000r / min for 15 minutes to obtain an aqueous solution of broken ginkgo pollen;

[0037] b. Introduce two probiotics, Lactobacillus rhamnosus and Lactobacillus acidophilus, into the MRS liquid seed medium, and culture them statically at 35°C for 12 hours to obtain two probiotic seed solutions;

[0038] c. get the broken ginkgo pollen aqueous solution of a step, mix according to the broken ginkgo pollen aqueous solution: sugar: sodium chloride: sodium glutamate=8:4:0.03:0.03 parts by weight, add 78 times the amount of deionized water Dissolve and sterilize at 115°C for 20 minutes to obtain a fermentation medium;

[0039] d. Take the two kinds of probiotic seed solutions in step b and combine them in a ratio of 1:1, then insert them into the fermentation medium ...

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Abstract

The invention provides a method for preparing probiotics and ginkgo pollen oral solution, which comprises the following steps of: a, putting fresh ginkgo pollen into water, and performing wall breaking treatment on the ginkgo pollen to obtain wall-broken ginkgo pollen; b, respectively inoculating two probiotics of lactobacillus rhamnosus and lactobacillus acidophilus into an MRS liquid seed culture medium, and standing for culture at the temperature of between 30 and 37 DEG C to obtain probiotic seed liquor; c, mixing the wall-broken ginkgo pollen I the step a, sugar, sodium chloride and sodium glutamate, dissolving by adding deionized water, and performing high temperature sterilization to obtain a fermentation medium; and d, combining the two kinds of probiotic seed liquor in the step 2 in a ratio of 1:1, inoculating into the fermentation medium in the step 3 according to 1 to 3 percent of the total inoculum size, sealing by a plastic film, standing for anaerobic culture until the pH of the fermentation medium is reduced to 3.0-3.5, filtering by using gauze, and filling under the aseptic condition to obtain the product.

Description

Technical field: [0001] The invention belongs to the technical field of food and beverages, and in particular relates to a preparation method of probiotic ginkgo pollen oral liquid which can improve human immunity and has health care function. technical background: [0002] With the improvement of people's health awareness, health food has attracted more and more attention. The development trend of this food industry is to use Chinese herbal medicine with the same source of medicine and food as well as products developed by microorganisms that improve gastrointestinal balance. Ginkgo biloba is a rare plant unique to our country and is known as the "living fossil" of the plant kingdom. The ginkgo tree is full of treasures, and its fruits, leaves and seedlings have important edible, medicinal and ornamental values. At present, many kinds of medicines and health care products have been developed by using the extracts of ginkgo leaves and ginkgo fruit (such as GBE761) at home ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L33/00
Inventor 杜方岭陈蕾蕾刘孝永孙欣裘纪莹祝清俊王未名
Owner 山东创新源农业技术开发有限公司
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