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Method for preparing micromolecular collagen peptide from fish scales by utilizing complex enzyme

A technology of collagen peptides and compound enzymes, which is applied in the field of preparation of small molecule protein peptides, to achieve the effect of wide substrate specificity

Inactive Publication Date: 2010-12-01
WUHAN AISHININA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the molecular weight distribution of collagen peptides largely depends on the combination and ratio of several proteases, the molecular weight of collagen peptides produced by the combination of several enzymes reported in the existing literature is mostly distributed between 2000 and 6000 Da, and the low Not yet reported at 1000 Da

Method used

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  • Method for preparing micromolecular collagen peptide from fish scales by utilizing complex enzyme

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Embodiment 1

[0019] Take 300g of dried fish scales, soak in dilute hydrochloric acid for 24 hours, wash until neutral, then soak in dilute sodium hydroxide for 24 hours, wash until neutral, add 1200mL of water, then add 0.3g of pepsin and 0.5g of papain and trypsin 0.7g, hydrolyze at a temperature of 40°C for 8 hours, then raise the temperature to 80°C, and keep the temperature for 30 minutes to inactivate the enzyme. After coarse filtration, membrane filtration with a molecular weight cut-off of 1000Da, vacuum concentration, and freeze-drying, the small molecule collagen peptide is obtained.

Embodiment 2

[0021] Take 500g of dried fish scales, soak in dilute hydrochloric acid for 24 hours, wash until neutral, then soak in dilute sodium hydroxide for 24 hours, wash until neutral, add 5000mL of water, then add 6g of pepsin, 4g of papain and pancreatic 10g of protease was hydrolyzed at 65°C for 5 hours, then the temperature was raised to 90°C and kept for 10 minutes to inactivate the enzyme. After coarse filtration, membrane filtration with a molecular weight cut-off of 1000Da, vacuum concentration, and freeze-drying, the small molecule collagen peptide is obtained.

Embodiment 3

[0023] Take 1000g of dried fish scales, soak them in dilute hydrochloric acid for 24 hours, wash until neutral, then soak them in dilute sodium hydroxide for 24 hours, wash until neutral, add 5000mL of water, then add 10g of pepsin, 6g of papain and pancreatic 4g of protease was hydrolyzed at 50°C for 6 hours, then the temperature was raised to 85°C and kept for 20 minutes to inactivate the enzyme. After coarse filtration, membrane filtration with a molecular weight cut-off of 1000Da, vacuum concentration, and freeze-drying, the small molecule collagen peptide is obtained.

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Abstract

The invention discloses a method for preparing micromolecular collagen peptide from fish scales by utilizing complex enzyme. The method comprises the steps of deashing and degreasing fish scales used as raw materials; adding complex enzyme mixed by pepsin, papain and trypsin for hydrolyzing, inactivating the enzyme, carrying out coarse filtering, film filtering, vacuum concentrating and freeze drying to obtain the micromolecular collagen peptide. The collagen peptide has the average molecular weight of 500-1,000Da, can be absorbed and utilized by human bodies easily, can be applied to cosmetic, healthcare products, foods, beverages and other industries, and has remarkable economic effect and social effect.

Description

(1) Technical field [0001] The invention relates to a method for preparing small molecule protein peptides, in particular to a method for preparing fish scale small molecule collagen peptides. (2) Technical background [0002] Collagen is an important functional protein, which is closely related to cell proliferation, differentiation, exercise, immunity, joint lubrication, wound healing, etc. Due to the special functions of collagen, its extracts have been widely used in medicine, food, daily chemical industry, biosynthesis and other industrial fields, such as manufacturing pharmaceutical capsules, surgical materials, health food, lighting gelatin, cosmetics, etc. Collagen peptides, widely used in cosmetics, health products, food and beverages as additives, are derived from collagen. The collagen in fish scales is as high as 45%, which contains a large amount of glycine, proline, hydroxyproline, and hydroxylysine, among which hydroxyproline and hydroxylysine are rarely foun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 王勇
Owner WUHAN AISHININA BIOTECH
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