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Method for preparing three-dimensional cell chip

A three-dimensional cell and chip technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of low reliability and accuracy of research results, and achieve high reliability and accuracy

Inactive Publication Date: 2010-11-24
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing cell chips mainly include the following categories: integrated microfluidic cell chips, micro-electroporation cell chips, and cellular immune chips. That is to say, only one side of the cell interacts with the external environment, and the living environment of the cell in the body is surrounded by stimulating factors that act on it. In addition, due to the two-dimensional culture of cells, especially for some special primary cultures Cells, some of the biological characteristics of the cells themselves have disappeared, and there are shortcomings in the reliability and accuracy of the research results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Preparation of three-dimensional mouse bladder smooth muscle cell chip

[0021] A method for preparing a three-dimensional cell chip, comprising the following specific steps:

[0022] The first step is to prepare a sol solution for forming a micropore array, mix a hydrogel macromer, a crosslinking agent and a hydrogel solvent in a mass ratio of 1:0.1:100 to form the first sol solution, and the hydrogel It is polyethylene glycol, the cross-linking agent is 2-hydroxy-methylphenylpropan-1-one, and the hydrogel solvent is a phosphate (PBS) buffer solution with a pH value of 7.5.

[0023] The second step is to prepare the sol solution mixed with the cell culture medium, mix the hydrogel macromer and the hydrogel solvent in a mass ratio of 1:200 to form the second sol solution, the hydrogel is collagen, and the hydrogel solvent for pure water,

[0024] The third step is to prepare the microwell array. Take 1ml of the first sol solution and place it on a methylate...

Embodiment 2

[0028] Example 2: Preparation of a three-dimensional sheep bone marrow pluripotent stromal cell chip

[0029] A method for preparing a three-dimensional cell chip, comprising the following specific steps:

[0030] The first step is to prepare a sol solution for forming a micropore array, mix the hydrogel monomer and the hydrogel solvent at a mass ratio of 1:200 to form the first sol solution, the hydrogel is collagen, and the hydrogel solvent is pure water,

[0031] The second step is to prepare the sol solution mixed with the cell culture medium, mix the hydrogel macromer, the cross-linking agent and the hydrogel solvent in a mass ratio of 1:0.05:100 to form the second sol solution, and the hydrogel is Hyaluronic acid, the cross-linking agent is dimethacrylic anhydride, and the hydrogel solvent is a phosphate (PBS) buffer solution with a pH value of 8.0,

[0032] The third step is to prepare the microwell array, take 2ml of the first sol solution and place it on a methylate...

Embodiment 3

[0036] Example 3: Preparation of a three-dimensional human neural stem cell chip

[0037] A method for preparing a three-dimensional cell chip, comprising the following specific steps:

[0038] The first step is to prepare a sol solution for forming a micropore array, mix a hydrogel macromer, a crosslinking agent and a hydrogel solvent in a mass ratio of 1:0.5:50 to form the first sol solution, and the hydrogel Chitosan, the cross-linking agent is β-sodium glycerophosphate, the hydrogel solvent is deionized water,

[0039] The second step is to prepare the sol solution mixed with the cell culture medium, mix the hydrogel macromer, the cross-linking agent and the hydrogel solvent in a mass ratio of 1:0.01:10 to form the second sol solution, and the hydrogel is Polyethylene glycol, cross-linking agent is 2-hydroxyl-methylphenyl propane-1-ketone, and hydrogel solvent is the phosphate (PBS) buffer solution that pH value is 5.7,

[0040] The third step is to prepare the microwell...

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PUM

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Abstract

The invention discloses a method for preparing a three-dimensional cell chip. The method comprises the following steps of: preparing sol solution for forming a micro-pore array; preparing sol solution mixed with cell culture fluid; preparing a micro-pore array; then preparing cell-containing cell culture fluid; preparing cell-gel suspension; and finally stamping cells into the micro-pore array. Or the method comprises the following steps of: preparing the sol solution for forming the micro-pore array; preparing the micro-pore array; then preparing the cell-containing cell culture fluid; and finally stamping the cells into the micro-pore array. In the cell chip prepared by the invention, the cells grow in a three-dimensional culture mode, so that the cell chip has the advantages of high reliability and high accuracy on research results.

Description

technical field [0001] The invention relates to the field of cell chip preparation, in particular to a method for preparing a three-dimensional cell chip. Background technique [0002] Biochip refers to immobilizing a large number of probe molecules on the support, then adding labeled sample molecules for hybridization, and obtaining the number and sequence information of sample molecules by detecting the signal intensity of each probe molecule, so as to realize the detection of cells and proteins. Accurate, rapid, and large-scale detection of genes, genes, and other biological components, the use of biochip technology to study cell metabolism mechanisms, bioelectrochemical preference conduction mechanisms, and intracellular environment have advantages that other traditional methods cannot compare with. At present, there have been a lot of reports on cell chips at home and abroad. The existing cell chip technology mainly uses microscopic technology or nanotechnology, and ado...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
Inventor 徐峰卢天健吴锦慧周丽宏
Owner XI AN JIAOTONG UNIV
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