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Microbial additive for silage fodder and preparation method thereof

A microbial additive and silage technology, applied in the field of silage, can solve side effects and other problems, and achieve the effects of making up for losses, shortening fermentation time, and prolonging storage time

Inactive Publication Date: 2010-11-10
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, antibiotics are widely used in animal treatment. While they play an important role in the prevention and treatment of animal diseases, they also bring certain side effects to animal husbandry and humans.

Method used

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  • Microbial additive for silage fodder and preparation method thereof
  • Microbial additive for silage fodder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: The present embodiment provides a kind of silage microbial additive, and using method thereof, fully mixes with apple pomace according to the following microbial formula: with fresh apple pomace as silage raw material, add plantaractobacillus respectively (number of bacteria is 5.0 ×10 9 ~1.0×10 10 cfu / mL) and Pediococcus pentosacea (the number of bacteria is 5.0×10 9 ~1.0×10 10 cfu / mL) liquid culture solution 0.1%~0.5% (dosage is calculated according to the amount of additives per ton of silage, for example, 1 ton of silage is added with 1~5kg of culture solution, the same below), aroma-producing yeast (the number of bacteria is 2.0×10 8 ~6.0×10 8 cfu / mL) liquid culture solution 0.1%~0.3%, Bacillus cereus (the number of bacteria is 1.0×10 8 ~5.0×10 8 cfu / mL) 0.1% to 0.2% liquid culture solution, fully stirred and mixed, put into a 40*80cm silage bag, squeeze out the air in the bag and seal it, and culture it at room temperature in the dark for 20 to ...

Embodiment 2

[0020] Embodiment 2: fully mix with the apple pomace according to the following bacterium formula: use fresh corn stalks without cobs as the silage raw material, add Lactobacillus plantarum respectively (the number of bacteria is 5.0 × 10 9 ~1.0×10 10 cfu / mL) and Pediococcus pentosacea (the number of bacteria is 5.0×10 9 ~1.0×10 10 cfu / mL) liquid culture solution 0.1%~0.5%, aroma yeast (the number of bacteria is 2.0×10 8 ~6.0×10 8 cfu / mL) liquid culture solution 0.1%~0.3%, Bacillus cereus (the number of bacteria is 1.0×10 8 ~5.0×10 8 cfu / mL) 0.1% to 0.2% liquid culture solution, fully stirred and mixed, put into a 40*80cm silage bag, squeeze out the air in the bag and seal it, and culture it at room temperature in the dark for 20 to 30 days. The main nutrient changes are shown in Table 2.

[0021] Table 2 Changes in main nutrients

[0022]

Embodiment 3

[0023] Embodiment 3: This embodiment provides a preparation method of microbial additives for silage. Lactobacillus plantarum, Pediococcus pentosaceae, Aroma-producing yeast, Issaccharomyces orientalis and Bacillus cereus are all prepared by liquid culture, wherein plant milk Bacillus and Pediococcus pentosaceae have the same medium, each liter of medium contains: 10g of peptone, 5g of yeast extract, 10g of beef extract, 20g of glucose, 5g of sodium acetate, 2g of diammonium citrate, 1mL of Tween 80, 0.58 of magnesium sulfate g, 0.05g of manganese sulfate, 2g of dipotassium hydrogen phosphate, 20g of agar, 1L of distilled water; the culture medium of Aroma-producing yeast and Issaccharomyces orientalis is the same, and each liter of liquid medium contains: yeast powder 2g, glucose 30g, dipotassium hydrogen phosphate 1.0 grams, 0.5 grams of magnesium sulfate, 2.0 grams of ammonium sulfate, 1 L of distilled water; each liter of Bacillus cereus liquid medium contains: 3 g of beef ...

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Abstract

The invention discloses a microbial additive for a silage fodder and a preparation method thereof. The microbial additive comprises lactobacillus plantarum, pediococcus pentosaceus, aroma-producing yeast, issatchenkia orientalis and bacillus cereus, and the microbial additive is prepared by a liquid culture method. The lactic acid content and acetic acid content of the composite silage fodder added with the microbial additive are improved, and simultaneously, the fodder does not contain propionic acid or butyric acid; compared with the fodder before ensiling, the fodder has the dry matter content which is increased on a small scale, and the ammoniacal nitrogen content which is increased but is low; and after the microbial additive is added, the CP content of the silage fodder is increased obviously, and is increased by 25 percent compared with the CP content of the fodder before the ensiling.

Description

technical field [0001] The invention relates to the technical field of silage, in particular to a microbial additive for silage and a preparation method thereof. Background technique [0002] At present, antibiotics are widely used in animal treatment. While they play an important role in the prevention and treatment of animal diseases, they also bring certain side effects to animal husbandry and humans. Therefore, at present, there are many studies on feed microorganisms. Feed microorganisms also become microecological additives, which can regulate the microbial balance of the gastrointestinal tract of animals, maintain the normal function of the gastrointestinal tract, and improve the health of the host, so as to achieve its goal. Disease prevention, treatment, growth promotion and other functions. Because it is a beneficial live bacteria preparation, it has the advantages that antibiotics do not have, such as non-toxicity, no side effects, no pollution, no residue, and n...

Claims

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Application Information

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IPC IPC(8): A23K1/16C12N1/00C12N1/20C12N1/14C12R1/25C12R1/01C12R1/085
Inventor 来航线肖健薛泉宏
Owner NORTHWEST A & F UNIV
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