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Preparation method and application of effective component of chrysanthemum

An effective component, chrysanthemum technology, applied in the field of traditional Chinese medicine extracts, can solve problems involving normal cells, side effects, etc., and achieve the effect of simple method and stable quality

Inactive Publication Date: 2010-10-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Preparation method and application of effective component of chrysanthemum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The preparation of embodiment one chrysanthemum effective component

[0012] After pulverizing 200g of chrysanthemum medicinal materials, add ethyl acetate and ethanol (1:0.8) (volume ratio) 1500ml, heat and extract once to obtain an extract, concentrate the extract into an extract, and use a reversed-phase silica gel column (ODS) to process it. Separation, first use 20% (volume ratio) ethanol solution as mobile phase, obtain eluent I, discard it, then change 90% (volume ratio) ethanol solution as mobile phase, obtain eluent II, eluent II The sample was obtained after concentration and drying; the obtained sample was further separated by preparative liquid chromatography, and the separation conditions of the preparative chromatography were as follows: the chromatographic column was a preparative column, the mobile phase was water and acetonitrile, and gradient elution was performed. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aque...

Embodiment 2

[0013] The preparation of embodiment two chrysanthemum effective components

[0014] After pulverizing 500g of chrysanthemum medicinal materials, add ethyl acetate and ethanol (1:1.2) (volume ratio) 5000ml, heat and extract 3 times to obtain an extract, concentrate the extract into an extract, and use a reversed-phase silica gel column (ODS) for its extraction. Separation, first use 40% (volume ratio) ethanol solution as mobile phase, obtain eluent I, discard it, then change 100% (volume ratio) ethanol solution as mobile phase, obtain eluent II, eluent II The sample was obtained after concentration and drying; the obtained sample was further separated by preparative liquid chromatography, and the separation conditions of the preparative chromatography were as follows: the chromatographic column was a preparative column, the mobile phase was water and acetonitrile, and gradient elution was performed. The gradient elution program was as follows: at 0 minutes, the mobile phase wa...

Embodiment 3

[0015] The preparation of embodiment three chrysanthemum effective components

[0016] After pulverizing 250g of chrysanthemum medicinal materials, add ethyl acetate and ethanol (1:1) (volume ratio) 2000ml, heat and extract twice to obtain an extract, concentrate the extract into an extract, and use a reversed-phase silica gel column (ODS) to carry out Separation, first use 30% (volume ratio) ethanol solution as mobile phase, obtain eluent I, discard it, then change 95% (volume ratio) ethanol solution as mobile phase, obtain eluent II, eluent II The sample was obtained after concentration and drying; the obtained sample was further separated by preparative liquid chromatography, and the separation conditions of the preparative chromatography were as follows: the chromatographic column was a preparative column, the mobile phase was water and acetonitrile, and gradient elution was performed. The gradient elution program was as follows: at 0 minutes, the mobile phase was 80% aque...

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Abstract

The invention provides a preparation method of an effective component of chrysanthemum, which comprises the following steps: extracting the medicinal material by heating, concentrating, separating by silica gel columns and eluting, and concentrating and drying eluent; and then continuing to separate by preparative liquid chromatography, collecting a solution, concentrating and drying the solution, and obtaining the effective component. The effective component of the chrysanthemum can be used as an active ingredient, and is added with pharmaceutically accepted excipients or carriers to be made into preparations according to methods recorded in pharmacy. The preparation form of the prepared drug is liquid preparations, solid preparations, capsule or pill preparations, and the administration method is oral or injection administration. The effective component of the chrysanthemum can be applied to drugs for preparing, treating and preventing tumor. The preparation method of the invention has simple operation and stable quality. The report that the chrysanthemum has the activity of liver tumor resistance does not exist in the prior art, so the invention provides a research foundation for novel chrysanthemum anti-tumor drugs.

Description

technical field [0001] The invention belongs to traditional Chinese medicine extracts, in particular to a preparation method for extracting effective components from chrysanthemums, and the application of the effective components in preparing medicines for treating and preventing tumors. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel i...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P35/00A61P1/16A61K133/00
Inventor 程翼宇瞿海斌吴斌
Owner ZHEJIANG UNIV
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