Method for cucumber hybrid seed production by honeybee pollination in net room
A technology for hybrid seed production and bee pollination, which is applied in the fields of botanical equipment and methods, applications, and plant genetic improvement, which can solve the problems of insufficient labor and high labor costs, and achieve the effects of increasing yield, increasing fruit setting rate, and ensuring purity.
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Embodiment 1
[0020] This embodiment is an embodiment of the method of the present invention, and three processing tests have been done in the planting mode of the parents.
[0021] The three treatments in this example are A1, A2 and A3 respectively, and the test materials for the three treatments are the cucumber hybrid combination provided by Syngenta, the female parent AF0309 is the female line material, and the male parent AF020 is the hermaphrodite plant material, the sowing date of the three treatments is August 20, and the planting period is September 2; the sowing period, the planting period, field management, and pest control of the three treatments are all conventional technical measures in the art and are all the same. The test steps are as follows:
[0022] 1. Construction of isolation net room
[0023] Build three isolation net rooms on the suitable land of soil fertility and do three processing uses respectively, and the construction of described three isolation net rooms is ...
Embodiment 2
[0043] The present embodiment is the test of the bee releasing time of the inventive method, and 5 treatments are set, respectively B1, B2, B3, B4, B5; the used test materials of the five treatments all select the cucumber hybrid combination provided by Syngenta , the female parent AF0309 is a female line material, and the male parent AF020 is a monoecious plant material, and adopts the planting method of processing A2 in Example 1. In order to determine the best time for releasing bees and pollination, the following tests are carried out, and other test steps are all the same as the implementation Example 1 is the same.
[0044] 1. Determination of release time
[0045] B1, B2, B3, B4, the bee release time of B5 were originally selected in 3 days, 4 days, 3 days, 4 days, 5 days before the female flowers of more than 7 nodes opened, and the beehives were moved into the net room.
[0046] 2. Test method
[0047] The test was carried out in five isolation net rooms respectivel...
Embodiment 9
[0078] Embodiment 9 is the embodiment of the inventive method
[0079] In Example 9, except that the male parent material used is the female line material CF090, and the following male flower induction treatment is performed, and the female parent is the same parent AF0309 used in Example 3, the rest of the steps are the same as in Example 3. When harvesting, investigate the number of results per plant, the number of seeds per fruit, the seed yield per plant, and the weight of 1,000-seed seeds. Each plot counts the yield respectively, and compares them with Example 3, see Table 7 and Table 8.
[0080] Male flower induction treatment: When the male parent seedlings grow to 3 leaves and 1 heart, and the diameter of the third true leaf is 2-4cm after flattening, use 250mg / L silver thiosulfate solution to spray the small watering can to grow Spots and young leaves are sprayed, and the young tissues of the whole plant should be fully moistened. Treat once every 3-4 days, and treat ...
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