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Method for extracting high-purity beta-glucan and whole oat flour from oat bran

A technology of oat bran and oat whole powder, which is applied in the direction of digestive system, metabolic diseases, food preparation, etc.

Inactive Publication Date: 2010-10-13
山西金绿禾燕麦研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem of improving the extraction of β-glucan from oat bran and effectively removing impurities therein, the present invention provides a method for extracting high-purity β-glucan and oat whole powder from oat bran

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, a kind of method for extracting high-purity β-glucan, whole oat powder from oat bran, comprises the steps:

[0023] 1) Stir 60-mesh defatted oat bran with water at 80° C. for 45 minutes, the weight ratio of oat bran to water is 1:10, and add 40 U / ml α-amylase at the same time. ;

[0024] 2) Filtrating the treated extract through a plate-and-frame filter to obtain filtered liquid and insoluble dietary fiber. Adjust the pH value of the obtained filtered liquid to 3.8, and obtain β-glucan liquid and oat protein isolate through continuous separation in a 10,000rpm high-speed centrifuge;

[0025] 3) Adjust the pH value of the isolated β-glucan liquid to 4.1, add 20 U / ml glucoamylase at 58°C, and stir for 30 minutes.

[0026] 4) Adjust the pH value of the liquid obtained in 3) to 4.8, add 10 U / ml pentosanase at 50° C., and stir for 45 minutes.

[0027] 5) Adjust the pH value of the liquid obtained in 4) to 3.8, add 20 U / ml pectinase at 40°C, and stir for 60 m...

Embodiment 2

[0034] Embodiment 2, a kind of method for extracting high-purity β-glucan, whole oat powder from oat bran, comprises the steps:

[0035] 1) Stir 80-mesh defatted oat bran with water at 80° C. for 55 minutes, the weight ratio of oat bran to water is 1:15, and add 50 U / ml α-amylase at the same time. ;

[0036] 2) Filtrating the treated extract through a plate-and-frame filter to obtain filtered liquid and insoluble dietary fiber. Adjust the pH value of the obtained filtered liquid to 4.2, and obtain β-glucan liquid and oat protein isolate through continuous separation in a 12000rpm high-speed centrifuge;

[0037] 3) Adjust the pH value of the separated β-glucan liquid to 4.5, add 30 U / ml glucoamylase at 60°C, and stir for 45 minutes.

[0038] 4) Adjust the pH value of the liquid obtained in 3) to 5.2, add 10-20 U / ml pentosanase at 52°C, and stir for 60 minutes.

[0039] 5) Adjust the pH value of the liquid obtained in 4) to 4.2, add 40 U / ml pectinase at 45°C, and stir for 90 mi...

Embodiment 3

[0046] Embodiment 3, a kind of method for extracting high-purity β-glucan, whole oat powder from oat bran, comprises the steps:

[0047] 1) Stir 100 meshes of defatted oat bran with water at 90° C. for 55 minutes, the weight ratio of oat bran to water is 1:20, and 60 U / ml α-amylase is added at the same time.

[0048] 2) Filtrating the treated extract through a plate-and-frame filter to obtain filtered liquid and insoluble dietary fiber. Adjust the pH value of the obtained filtered liquid to 4.4, and obtain β-glucan liquid and oat protein isolate through continuous separation in a 12000rpm high-speed centrifuge;

[0049] 3) Adjust the pH value of the separated β-glucan liquid to 4.5, add 30 U / ml glucoamylase at 60°C, and stir for 45 minutes.

[0050] 4) Adjust the pH value of the liquid obtained in 3) to 5.0, add 15 U / ml pentosanase at 52°C, and stir for 60 minutes.

[0051] 5) Adjust the pH value of the liquid obtained in 4) to 4.2, add 35 U / ml pectinase at 45°C, and stir fo...

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Abstract

The invention relates to a technology for extracting beta-glucan from oat bran, in particular to a method for extracting high-purity beta-glucan and whole oat flour from oat bran, aiming to solve the problem on how to improve the efficiency of extracting beta-glucan from oat bran and effectively remove impurities in the beta-glucan. The method comprises the following steps of: adding water to defatted oat bran, stirring and adding alpha-amylase; filtering and centrifuging obtained filter liquid to obtain beta-glucan liquid and oat isolated protein; adding diastatic enzymes, pentosanase, pectinase and neutral protease, inactivating enzyme and filtering by using a microfiltration membrane; treating by using ion exchange resin; and concentrating by using an ultrafiltration membrane to obtain the beta-glucan. The obtained beta-glucan has an extremely high purity capable of reaching higher than 90-97 percent, and whole oat flour can be obtained from separated fibers, and the like.

Description

technical field [0001] The invention relates to a technology for extracting beta-glucan from oat bran, in particular to a method for extracting high-purity beta-glucan and whole oat powder from oat bran. Background technique [0002] Oat, as a miscellaneous grain crop, has been paid more and more attention in recent years, which is due to the awareness of the health function of oat at home and abroad. The functions of oats in lowering blood fat and preventing cardiovascular and cerebrovascular diseases have been recognized all over the world, and the primary functional factor is considered to be β-glucan, a soluble dietary fiber rich in oats. β-glucan is a linear polysaccharide formed by the linkage of glucose through β-1,3 and β-1,4 glycosidic linkages, which is mainly concentrated in the aleurone layer of oat drum skin. Oat β-glucan has multiple functions, such as lowering blood lipids, controlling blood sugar, enhancing immunity, preventing constipation, improving intest...

Claims

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Application Information

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IPC IPC(8): C08B37/02A23L1/09A61P1/00A61P1/10A61P3/06A61P3/10A61P37/04A23L33/125A23L33/21
Inventor 田向东田伟民陈志刚
Owner 山西金绿禾燕麦研究所
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