Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing active ingredient of yerbadetajo herb and application

A technology of active ingredient and Eclipta chinensis, which is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, can solve problems such as side reactions, involving normal cells, etc., and achieve the effects of stable quality and simple method.

Inactive Publication Date: 2010-10-13
ZHEJIANG UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing active ingredient of yerbadetajo herb and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 Preparation of Eclipta prostrata effective components

[0012] After crushing 200g of Eclipta prostrata, 1500ml of ethyl acetate and ethanol (1:0.8) (volume ratio) are added, and the extract is heated and extracted once. The extract is concentrated into an extract, and the reverse phase silica gel column (ODS) For separation, first use 20% (volume ratio) ethanol solution as the mobile phase to obtain eluent I, discard it, and then change the 90% (volume ratio) ethanol solution as the mobile phase to obtain eluent II. The liquid II is concentrated and dried to obtain a sample; the obtained sample is continued to be separated by preparative liquid chromatography, and the separation conditions of the preparative chromatogram: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, gradient elution. The gradient elution procedure is as follows: at 0 minutes, the mobile phase is 80% aqueous solution and 20% acetonitrile solution...

Embodiment 2

[0013] Example two Preparation of Eclipta prostrata effective components

[0014] After crushing 500g of Eclipta prostrata, 5000ml of ethyl acetate and ethanol (1:1.2) (volume ratio) are added, and the extract is heated and extracted 3 times. The extract is concentrated into an extract, and the reverse phase silica gel column (ODS) For separation, first use 40% (volume ratio) ethanol solution as the mobile phase to obtain eluent I, discard it, and then change to 100% (volume ratio) ethanol solution as the mobile phase to obtain eluent II. The liquid II is concentrated and dried to obtain a sample; the sample obtained is continued to be separated by preparative liquid chromatography, and the separation conditions of the preparative chromatogram: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, gradient elution. The gradient elution procedure is as follows: at 0 minutes, the mobile phase is 80% aqueous solution and 20% acetonitrile sol...

Embodiment 3

[0015] Example Preparation of effective components of Eclipta prostrata

[0016] After pulverizing 250g of Eclipta prostrata, 2000ml of ethyl acetate and ethanol (1:1) (volume ratio) are added, and the extract is heated and extracted twice. The extract is concentrated into an extract, and the reversed-phase silica gel column (ODS) For separation, first use 30% (volume ratio) ethanol solution as the mobile phase to obtain eluent I, discard it, and then change the 95% (volume ratio) ethanol solution as the mobile phase to obtain eluent II. The liquid II is concentrated and dried to obtain a sample; the obtained sample is continued to be separated by preparative liquid chromatography, and the separation conditions of the preparative chromatogram: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, gradient elution. The gradient elution procedure is as follows: at 0 minutes, the mobile phase is 80% aqueous solution and 20% acetonitrile solu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preparing an active ingredient of yerbadetajo herb, which comprises the following steps of: heating and extracting medicinal materials, concentrating the extract, separating the extract with a silica gel column, eluting the extract, concentrating and drying the elute, continuously separating the elute by using a prepared liquid phase chromatograph, collecting the solution, and concentrating and drying the solution to obtain the active ingredient. The active ingredient of the yerbadetajo herb can be used as an active ingredient, and pharmaceutically acceptable medicinal excipient or carrier is added into the active ingredient to prepare a preparation according to a method recorded on pharmaceutics. The preparation form of the prepared medicament is liquid, solid, capsules or pills, and the administration mode is oral administration or injection administration. The active ingredient of the yerbadetajo herb provided by the invention can be applied in preparing medicaments for treating and preventing tumor. The preparation method has simple and convenient operation and stable quality, and provides a research foundation for researching and developing new anti-tumor medicaments of the yerbadetajo herb because liver tumor activity resistance of the yerbadetajo herb is not yet reported in the prior art.

Description

Technical field [0001] The invention belongs to a traditional Chinese medicine extract, and specifically relates to a preparation method of extracting effective components from Eclipta prostrata, and the application of the effective components in preparing medicines for treating and preventing tumors. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumors are the most serious type of diseases that endanger human health. At present, the treatment of malignant tumors in the industry is mainly surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genetic toxicity of botanical drugs is not obvious. Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation. Moreover, Chinese medicine also plays an important role in the adjuvant treatment of tumors. Paclitaxel ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/28A61P35/00
Inventor 程翼宇瞿海斌吴斌
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com