Millettia extract and preparation method and application thereof

Abstract

The invention discloses a millettia extract and a preparation method and the application thereof. 45 to 55 percent of ethanol is added into millettia medicine to be extracted and is loaded to a macroporous absorption resin column, and is eluted sequentially by water, 10 to 30 percent of ethanol and 65 to 75 percent of ethanol. 65 to 75 percent of ethanol is used for eluting a part, the ethanol is recovered and evaporated to dryness to obtain the millettia extract. Based on a tradition preparation method, the preparation method of the millettia extract designs reasonably and obtains an optimized production process combination, and the millettia extract obtained by the preparation method has high yield and more singificaly improves the functions of leukocytes compared with the millettia extract obtained by the traditional preparation method.

Description

technical field [0001] The present invention relates to an extract of Spatholobus Spatholobus and its preparation method and application. Background technique [0002] Tumors are usually associated with varying degrees of anemia, and the current treatment for malignant tumors is mainly based on surgical excision, radiotherapy, and chemotherapy, and almost all anticancer drugs affect normal cells while acting on cancer cells, so tumor patients after radiotherapy It often causes bone marrow suppression, manifested as a decrease in white blood cells, red blood cells, and platelets in varying degrees, and the most obvious decrease is white blood cells. Currently, there are Qiangli Shengbai Tablets and Ruibai tablets in clinical use, but due to factors such as high toxicity and high price, their range of application is limited and they cannot really meet the clinical needs. Finding new myelosuppressive drugs with good therapeutic effect, low toxicity and relatively cheap price h...

AI Technical Summary

Problems solved by technology

[0005] The main components of Caulis Spatholobus are flavonoids, phenols, triterpenoids and steroidal compounds. There have been some reports on the extraction, purification and activity of its active parts: Patent CN101347496A uses a mixed solvent of ethyl acetate and ethanol to extract , the extract is passed through a normal-phase silica gel column, eluted with petroleum ether, ethyl acetate, chloroform, etc., and finally preparative liquid chromatography is used for elution with acetonitrile-water, and the obtained components are used for malignant tumors. This method uses chloroform, Toxic solvents such as acetonitrile, and the application of precision instruments such as liquid preparation, are not suitable for industrialized large-scale production; patent CN1939396A is to moisten the medicinal material of Caulis Spatholobus, then extract it with water or 80% ethanol under reflux, and the extract is adsorbed by polyamide chromatography column Afterwards, different concentrations of ethanol were eluted, and the obtained components were used for malignant tumors. The disadvantage of this method is that the extraction time is too long and the steps are cumbersome; there are also reports in the literature that water and / or ethanol are used to reflux to extract Caulis Spatholobus, and the extract is made of macroporous resin After adsorption, it is eluted with water and / or ethanol to obtain the extract of Spatholobus Spatholobus, which is then refined with macroporous resin. During the process, the extraction solvent is general reagent water or high-concentration ethanol, and the extraction target substance is too extensive. In order to cause inconvenience for the subsequent refinement, after the extract was absorbed by the macroporous resin, it was eluted with full gradient water-ethanol. The process was relatively complicated, and the obtained Caulis Spatholobus extract had no obvious effect on increasing white blood cells.

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