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Method for culturing Metarhizium anisopliae

A technology of Metarhizium anisopliae and culture medium, which is applied in the field of cultivating Metarhizium anisopliae, can solve the problems of hindering the production process of Metarhizium anisopliae, low spore content of the bacterial agent, and long production cycle, so as to reduce the cultivation time and use Space, high production efficiency, economical and practical production process

Inactive Publication Date: 2010-09-29
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the cultivation methods for Metarhizium anisopliae generally have long production cycle, low spore content and high cost, which hinder the production process of Metarhizium anisopliae.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. First-level plate culture:

[0028] Inoculate the isolated and purified strains on a PDA medium plate, put them in a 28°C incubator, and cultivate them for 7 to 10 days. When the mycelia are all over the plates and produce spores, transfer them to shake flasks for culture;

[0029] The composition of each 1000ml PDA medium: potato 200g, peptone 10g, glucose 20g, agar 20g, water 1000ml.

[0030] 2. Liquid seed culture:

[0031] In a 1000ml triangular flask, put 400ml of liquid seed culture medium into it, insert the plate bacteria, and the inoculation amount is 1000ml triangular flask to connect 1 / 6 of the well-cultured first-level plate bacteria (petri dish specification 90mm), after inoculation, place Cultivate in a constant temperature shaker (28°C, 180 rpm) for 3 to 5 days;

[0032] The composition of each 1000ml liquid seed medium: 20g corn flour, 40g bean cake powder, 2.5g peptone, 0.5g KH 2 PO 4 , 0.5gMgSO 4 , 0.5gKCl, 0.01gFeSO 4 ·7H 2 O and 1000ml of w...

Embodiment 2

[0050] 1. Strain cultivation on a first-level flat plate: the same as the method described in Example 1.

[0051] 2. Liquid seed culture: the same as the method described in Example 1.

[0052] 3. Liquid fermentation production: the method is the same as that described in Example 1, except that the cultivation time is 68 hours.

[0053] 4. Solid fermentation production

[0054] The solid fermentation method is the same as the method described in Example 1, except that the cultivation time of the a stage is 3 days, and the solid culture material is composed differently, as follows:

[0055] Composition of solid compost: bagasse, wheat bran, corn flour, KH 2 PO 4 , MgSO 4 , KCl and water; its mass and number ratio is: 350 parts of bagasse: 180 parts of bran: 120 parts of corn flour: 0.5 part of KH 2 PO 4 : 0.5 parts of MgSO 4 : 0.5 parts of KCl: 350 parts of water; natural pH, steam sterilization at 121°C for 60 minutes;

[0056] 5. Drying of fermentation products

[00...

Embodiment 3

[0060] 1. Strain cultivation on a first-level flat plate: the same as the method described in Example 1.

[0061] 2. Liquid seed culture: the same as the method described in Example 1.

[0062] 3. Liquid fermentation production: the same method as described in Example 1, except that the cultivation time was 72 hours.

[0063] 4. Solid fermentation production

[0064] The solid fermentation method is the same as the method described in Example 1, except that the solid compost composition is different, as follows:

[0065] Composition of solid compost: bagasse, wheat bran, corn flour, KH 2 PO 4 , MgSO 4 , KCl and water; its mass and number ratio is: 400 parts of bagasse: 200 parts of bran: 90 parts of corn flour: 0.5 part of KH 2 PO 4 : 0.5 parts of MgSO 4 : 0.5 parts of KCl: 400 parts of water; natural pH, steam sterilization at 121°C for 60 minutes;

[0066] 5. Drying of fermentation products

[0067] The method is the same as that described in Example 1.

[0068] Re...

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Abstract

The invention discloses a method for culturing Metarhizium anisopliae, which comprises the following steps of: 1) performing liquid fermentation culture on the Metarhizium anisopliae by using a fluid nutrient medium, and marking all substances in a container as liquid fermentation products; and 2) inoculating the liquid fermentation products into a solid fermentation medium for solid fermentation culture to obtain the Metarhizium anisopliae. The method is a method for culturing the Metarhizium anisopliae by liquid-solid dual-phase fermentation, and combines liquid fermentation tank production with solid fermentation production. In liquid submerged fermentation, the medium adopted takes bagasse as a main carrier, three cheap and readily available agricultural products are taken as raw materials, and the cost is greatly reduced.

Description

technical field [0001] The invention relates to a method for cultivating Metarhizium anisopliae. Background technique [0002] The extensive use of chemical pesticides has caused serious impacts on the environment and ecology, and people have paid more and more attention to biological control. The use of entomopathogenic microorganisms to control pests is one of the important means of biological control. There are many kinds of entomogenic fungi, complex metabolic types, and they are playing an increasingly important role in the biological control of pests due to their safety and effectiveness, significant epidemic potential, and easy mass production. Metarhizium anisopliae is a worldwide distribution of insecticidal fungi, which can parasitize about 200 species of insects, mites and nematodes in 30 families of 8 orders. The use of Metarhizium anisopliae to control pests has been studied for a hundred years. It has strong pathogenicity and good effect, and is non-toxic to h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 黄俊生梁昌聪杨腊英代鹏黄志刘磊王国芬谢玉萍
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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