Method for preparing dietary fiber by adopting corn bran hydrolyzed by multifunctional enzyme

A multifunctional enzyme and dietary fiber technology, which is applied in the field of corn deep processing, can solve the problems of water-soluble dietary fiber loss, low water holding capacity and expansion force, and achieve the effects of increasing added value, saving energy, and simple process

Inactive Publication Date: 2010-09-29
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method is characterized by low cost, but the acid-base treatment process causes a large amount of water-soluble dietary fiber to be lost, and the product has low water holding capacity and swelling power

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Add 70 L of 0.6% NaOH to 1 kg of 80-mesh corn husks and soak for 1.5 hours;

[0016] 2) Sterilize the soaked material under high pressure at 0.105PKa at 121°C for 50min;

[0017] 3) After cooling the soaking solution to 50°C, adjust the pH to 8, add 20ul of multifunctional enzyme (APE1547) per ml of soaking solution to hydrolyze the ester bond and peptide bond on the cell wall, and put it in a shaker at 50°C for 2 hours. ; Centrifuge at 5500r for 10min;

[0018] 4) Take the precipitate, heat it at 70°C with a solid-liquid ratio of 1:6 (W / V), add hydrogen peroxide with a final concentration of 0.3%, decolorize for 45 minutes, wash with water until it is non-oxidizing, and pH=9.0

[0019] 5) Dried below 60°C or naturally dried, crushed and passed through a 80-100 mesh sieve to obtain dietary fiber.

[0020] Its expansion force reaches 14.5ml / g, and its water holding capacity is 11g / g.

Embodiment 2

[0022] 1) Add 650L of 0.6% NaOH to 100kg of 80-mesh corn husks, and soak for 1.5 hours in the dark;

[0023] 2) Sterilize the soaked material under high pressure at 0.105PKa at 121°C for 40min;

[0024] 3) After cooling the soaking solution to 50°C, adjust the pH to 8, add 20ul of multifunctional enzyme (APE1547) per ml of soaking solution to hydrolyze the ester bond and peptide bond on the cell wall, and put it in a shaker at 50°C for 2 hours. ; Centrifuge at 5500r for 10min;

[0025] 4) Take the precipitate, solid-liquid ratio 1:6 (W / V), PH=8.5, heat at 90°C, add hydrogen peroxide with a final concentration of 0.3%, decolorize for 30 minutes, wash with water until non-oxidizing,

[0026] 5) Dried below 60°C or naturally dried, crushed and passed through a 80-100 mesh sieve to obtain dietary fiber.

[0027] Its expansion force reaches 12ml / g, and its water holding capacity is above 10g / g.

Embodiment 3

[0029] 1) Add 70 L of 0.6% NaOH to 10 kg of 80-mesh corn husks, and soak for 1.5 hours in the dark;

[0030] 2) Sterilize the soaked material under high pressure at 0.105PKa at 121°C for 30 minutes;

[0031] 3) After cooling the soaking solution to 50°C, adjust the pH to 8, add 20ul of multifunctional enzyme (APE1547) per ml of soaking solution to hydrolyze the ester bond and peptide bond on the cell wall, and put it in a shaker for 2 hours at 60°C ; Centrifuge at 5500r for 10min;

[0032] 4) Take the precipitate, heat it at 70°C with a solid-liquid ratio of 1:6 (W / V), add hydrogen peroxide with a final concentration of 0.3%, decolorize for 50 minutes, wash with water until it is non-oxidizing, and pH=8.5

[0033] 5) Dried below 60°C or naturally dried, crushed and passed through a 80-100 mesh sieve to obtain dietary fiber.

[0034] Its swelling force reaches 10ml / g, and its water holding capacity is 9g / g.

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PUM

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Abstract

The invention provides a preparation method of dietary fiber by adopting corn bran hydrolyzed by a multifunctional enzyme. The preparation method comprises the following steps of: adding the corn bran into NaOH and soaking darkly, sterilizing at high pressure, adding the multifunctional enzyme with peptidase and esterase activities for hydrolyzing ester bonds and peptide bonds on the cell wall, centrifugally taking precipitate, decoloring and rinsing, drying at the temperature below 60 DEG C, crushing and screening by adopting a 80-100 mesh sieve to obtain the dietary fiber. The dietary fiber which has the expansion force up to 10-15ml/g and the water-holding capacity of 9-11g/g and is superior to other methods is prepared by utilizing the corn bran as a by-product in the process of grain processing or fuel ethanol production and the hydrolysis of the multifunctional enzyme with peptidase and esterase activities. The invention utilizes the enzyme, has simple process, saves time and energy and increases the additional value of the corn by-products.

Description

technical field [0001] The invention relates to a production method of dietary fiber, and in particular provides a method for preparing dietary fiber by hydrolyzing corn husks with a multifunctional enzyme. The dietary fiber is obtained by using a multifunctional enzyme expressed by engineering bacteria, which belongs to the technical field of corn deep processing. Background technique [0002] Cereal plant cell walls mainly contain plant polysaccharides; proteins, lignin and a small amount of phenolic compounds. In recent years, researchers have used amylase and protease to remove starch and protein remaining in corn bran, then used sodium hydroxide solution to extract hemicellulose, and then used acid and alkali to treat cellulose, hemicellulose, and lignin. Different solubilities can be used to separate them to obtain different corn dietary fiber products, and the performance of the products is very different. Literature research shows that domestic use of corn bran is l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/28A23L1/10A23L1/308A23L33/21
Inventor 张桂荣王志宏李亚芹
Owner JILIN UNIV
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