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Asparagus parent breeding method

A technology of asparagus and parent, which is applied in the field of parent propagation of herbaceous plants, can solve the problems of low rooting rate, poor root quality, and low survival rate of transplanting, and achieve the effects of convenient material collection, increased proliferation speed, and improved proliferation efficiency

Inactive Publication Date: 2011-09-07
北京农科院种业科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention mainly solves the problems encountered in the process of asparagus organ tissue culture, such as low rooting rate, poor root quality, low transplanting survival rate, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The parental propagation method of asparagus of the present invention is to select high-quality asparagus and cut it into a stem section with a well-developed axillary bud, and then after disinfection and washing pretreatment, the asparagus is cultured in a medium solution, and the reproduction can reach the transplanting standard. The process of asparagus seedlings.

[0024] The composition and preparation of the basic medium solution:

[0025] The components of the basic medium solution are as follows: NH per liter of solution 4 NO 3 1650mg, KNO 3 1900mg, CaCl 2 ·2H 2 O 440mg, MgSO 4 ·7H 2 O 370mg, KH 2 PO 4 170mg, KI 0.83mg, H 3 BO 3 6.2mg, MnSO 4 ·4H 2 O 22.3mg, ZnSO 4 ·7H 2 O8.6mg, Na 2 MoO 4 ·2H 2 O 0.25mg, CuSO 4 ·5H 2 O 0.025mg, CoCl 2 ·6H 2 O0.025mg, Na 2 EDTA (disodium ethylenediaminetetraacetic acid) 37.3mg, FeSO 4 ·7H 2 O 27.8mg, VB1 (vitamin B1) 0.4mg, VB6 (vitamin B6) 0.5mg, glycine 2.0mg, niacin 0.5mg, inositol 100mg, agar 5g, sucrose 30g.

[0026] The specific ...

Embodiment 2

[0034] The second embodiment: (the parts not stated in this embodiment are the same as the first embodiment)

[0035] The asparagus parent tissue propagation method of the present invention, the asparagus parent tissue propagation method of the present invention, is to select high-quality asparagus and cut into stems with a well-developed axillary bud, and then perform disinfection and washing pretreatment in a medium solution Cultivate and reproduce the process of transplanting standard asparagus seedlings.

[0036] The composition and preparation of the basic medium solution:

[0037] The components of the basic medium solution are as follows: Each liter of the basic medium solution contains: NH 4 NO 3 1400mg, KNO 3 2000mg, CaCl 2 ·2H 2 O 600mg, MgSO 4 ·7H 2 O250mg, KH 2 PO 4 260mg, KI 1.2mg, H 3 BO 3 5mg, MnSO 4 ·4H 2 O 15mg, ZnSO 4 ·7H 2 O 10mg, Na 2 MoO 4 ·2H 2 O 0.4mg, CuSO 4 ·5H 2 O 0.04mg, CoCl 2 ·6H 2 O 0.04mg, Na 2 EDTA 25mg, FeSO 4 ·7H 2 O 40mg, VB1 0.5mg, VB6 0.6mg, glyci...

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Abstract

The invention relates to asparagus parent breeding method, mainly solving the problems that rooting rate is low, root quality is poor and the survive rate of the regeneration plant is low in asparagus organ tissue culture process. The method includes the process that high quality asparagus stem section is selected, culture is carried out in culture medium solution, and asparagus seedling meeting transplant standard is bred. The culture medium solution is confected by the way that hormones corresponding to various culture stages are added on the basis of the basic culture medium solution, the culture includes induction culture, propagation culture, rooting pre-differentiation culture and rooting culture sequentially. Compared with the existing organ culture, the invention has high propagation rate, rooting rate reaches 100%, root system quality is good, and differentiation rate of strong fleshy tap root is improved to 96%, thus ensuring survival rate after transplantation.

Description

Technical field [0001] The present invention belongs to the field of parental propagation of herbaceous plants, specifically asparagus parental propagation technology. Background technique [0002] The scientific name of asparagus is asparagus, which is a perennial perennial herb of the genus Asparagus in the Liliaceae family. It is a typical dioecious plant. The reproduction of its hybrid parents can only adopt asexual reproduction methods. [0003] The asexual propagation methods of asparagus include the field natural ramification method and the tissue culture method. [0004] The field natural ramification method is to dig out the dormant roots from the field when the parent plants have grown for more than 3 years, divide them into 3 to 5 spore groups according to their natural growth state, and then plant them back in the field to achieve The purpose of proliferation. This method has very low reproduction efficiency and is not suitable for large-scale parental reproduction. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 尹俊玉叶劲松赵卫星
Owner 北京农科院种业科技有限公司
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