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Use of ethanol plant by-products for yeast propagation

A by-product, ethanol technology, applied in the direction of fermentation, biofuel, etc., to achieve good utilization effect

Active Publication Date: 2015-11-25
ARCHER DANIELS MIDLAND CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

OAG may also need to be disposed of as waste

Method used

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  • Use of ethanol plant by-products for yeast propagation
  • Use of ethanol plant by-products for yeast propagation
  • Use of ethanol plant by-products for yeast propagation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 reports batch fermentor cultivation of Saccharomyces cerevisiae utilizing organic acids and glycerol obtained as by-products of evaporation from reflux from a dry mill ethanol production facility. Batch yeast propagation was performed in two 3.5L fermentors. Concentrate OAG to between 5 and 10 times the original OAG concentration.

[0051] The pH of the culture was adjusted to 4.4 using sodium hydroxide. Nitrogen was also added to the mixture as an ammonium salt until the nitrogen concentration (as ammonia) was 2.5 g / L. The inoculation ratio was 1% v / v.

[0052] The results are shown in Figure 4 middle. Organic acids are preferred metabolites of yeast. The preference for glycerol is secondary. Organic acid depletion is indicated by a significant increase in dissolved oxygen concentration, which is not shown in the figure.

[0053] Cultures reach approximately 9 x 10 in 24 hours 8 cells / ml and a carbon source of 15-17 g / L was used during this time. La...

Embodiment 2

[0055] Example 2 reports the cultivation of Saccharomyces cerevisiae utilizing organic acids and glycerol obtained as by-products of evaporation from reflux from a dry mill ethanol production facility. Fermentation was initially carried out as a batch process, and then when more than 5 x 10 was considered sufficient 8 The yeast mass was then moved to a continuous fermenter.

[0056] After being moved to continuous fermentation mode, the fermentation quickly reached steady state conditions with a retention time of 5 to 6 hours. like Figure 5 As shown, the culture density, as expressed by optical density (OD at 660 nm) or by viable yeast cell counts, remained stable over the duration of the continuous feed / drop phase, which was maintained for 90 hours. About 16 fermentor volumes were produced during this period. The average yeast concentration during the continuous phase was 1.2 x 10 9 cells / ml.

[0057] Image 6 The consistency of the fermentation described in this examp...

Embodiment 3

[0059] Example 3 shows yeast culture utilizing organic acids according to another embodiment of the present invention. The conditions were the same as in Example 2, but the organic acid was not concentrated. The results of this example are shown in Figure 7 . Cultivation was limited by low substrate concentration, as indicated by low optical density and yeast concentration when compared to the results of Example 2.

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Abstract

Embodiments of the invention relate, for example, to methods for reducing the load of organic acids and glycerol in water recycled to the fermentation process. Organic acids and glycerol produced during ethanol fermentation are used as a replacement for carbohydrates for propagation of yeast. The yeast may be sold as a feed product or used in subsequent fermentation.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS [0002] This application claims the benefit of US Patent Application Serial No. 61 / 001,714, filed November 2, 2007. US Patent Application Serial No. 61 / 001,714 is incorporated by reference as if fully rewritten herein. [0003] Statement on Federally Funded Research [0004] none. Field of Invention [0005] The present teachings relate to, but are not limited to, the fields of ethanol fermentation, yeast production, and reducing the amount of waste from those processes. Background of the Invention [0006] The following includes information that can be used to understand the present teachings. This is not an admission that any of the information or material provided herein is prior art with respect to the subject matter presently described or claimed, nor is it an admission that any publication or document cited, specifically or implicitly, is current technology. [0007] Background of the prior art [0008] Figure 1 ill...

Claims

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Application Information

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IPC IPC(8): C12P7/06C12P7/14
CPCC12P7/14Y02E50/17Y02E50/10
Inventor 加迪·斯泰纳
Owner ARCHER DANIELS MIDLAND CO
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