Scid mouse model of electromagnetic radiation carcinogenesis and constructing method thereof
A technology of electromagnetic radiation and mouse models, applied in biochemical equipment and methods, electric/wave energy treatment of enzymes, treatment of microorganisms with electric/wave energy, etc., to achieve simple steps, significant effects, and prevent long-term harm
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Embodiment 1
[0024] (1) Culture and passage of NIH / 3T3 cells
[0025] NIH / 3T3 cells were incubated at 37°C, 5% CO 2 Culture in the incubator with DMEM culture medium. When the cells grow and proliferate to form a single layer of cells and expand to confluence, suck out the old culture medium, add 0.03% Versene solution to wash once, suck out the washing liquid, and then add trypsin digestion solution for digestion at 37°C. At the same time, observe the digested cells under an inverted microscope. When the original adherent cells gradually tend to be round, the cytoplasm retracts, the gap increases, and the cells are no longer connected into sheets, stop the digestion immediately, discard the digestion solution and add fresh Use a pipette to blow the digested cells into a single-cell suspension, aspirate the cell suspension and divide it into 3 culture bottles, add the culture medium and tighten the bottle cap, put in CO 2 Continue culturing in the incubator;
[0026] (2) Exposure experim...
Embodiment 2
[0034] The power density of electromagnetic radiation is 50W / m in the step (2) 2 , other steps are with embodiment 1. Both Scid mice developed tumors.
Embodiment 3
[0036] The power density of electromagnetic radiation in the step (2) is 70W / m 2 , other steps are with embodiment 1. Both Scid mice developed tumors.
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