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Industrialized one-step method for extracting housefly larvae fat

A technology of pure home and larvae, applied in the direction of producing fat, fat oil/fat production, etc., can solve problems such as restricting the development of the industry, and achieve the effects of being beneficial to environmental protection, short production cycle and excellent quality

Inactive Publication Date: 2010-09-22
本溪恒远生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned drawbacks existing in the prior art have seriously restricted the development of this industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The present invention studies the lipid content rate in each stage of the housefly larva life history, and finds that the lipid content rate is the highest in the early stage of pupae. It was therefore determined to harvest larvae at the prepupal stage. After harvesting, let it stand for 2h-6h, so as to discharge the dirt in the body.

[0016] In order to completely remove the residual feed mixed in the larvae, the larvae are placed in a container with a density of 1.08g / cm 3 -1.09g / cm 3 In the NaCl solution, using the difference in density between the residue and the larvae, the remaining feed sinks and the mature larvae float up. The larvae in the upper layer were picked up, placed in the fourth-stage cleaning tank, and countercurrently washed in the fourth-stage to remove NaCl and body surface secretions on the body surface.

[0017] Insect phenoloxidase (phenoloxidase, PO) is an important immune defense factor, which usually exists in the hemolymph in the form of...

Embodiment 2

[0020] Larvae that had grown to the prepupal stage were isolated from the larval rearing box. The larvae are harvested from the early pupal stage to the early pupal stage, and left to stand for 4 hours for bowel cleansing; the larvae are placed in a density of 1.09g / cm 3 In the NaCl solution, the mixed residual feed was precipitated. Harvest the cleaned larvae in the upper layer and wash them with four-stage clear water to remove NaCl and secretions on the body surface. Harvested larvae were denatured at 100°C. Grind the denatured larvae into whole pulp. Dry the whole worm pulp at 100°C. The crushed and dried material has a particle size of 60 mesh sieve. with supercritical CO 2 The extraction equipment extracts the insect fat. The pressure of the extraction kettle is 25MPa, the temperature is 40°C, the time is 2.5h, CO 2 The flow rate is 24kg / h. Extraction with supercritical CO 2 The separation conditions of the solvent are as follows: the pressure of the separation ...

Embodiment 3

[0022] Larvae that had grown to the prepupal stage were isolated from the larval rearing box. The larvae are harvested from the pre-pupal stage to the early pupal stage, and left to stand for 6 hours for bowel cleansing; the larvae are placed in a density of 1.08g / cm 3 In the NaCl solution, the mixed residual feed was precipitated. Harvest the cleaned larvae in the upper layer and wash them with four-stage clear water to remove NaCl and secretions on the body surface. Harvested larvae were denatured at 90°C. Grind the denatured larvae into whole pulp. Dry the whole worm pulp at 90°C. The crushed and dried material has a particle size of 80 mesh sieve. with supercritical CO 2 The extraction equipment extracts the insect fat. The pressure of the extraction kettle is 30MPa, the temperature is 45°C, the time is 4h, CO 2 The flow rate is 30kg / h. The pressure of the separation tank I is 12MPa and the temperature is 45°C, the pressure of the separation tank II is 7MPa and the...

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Abstract

The invention provides an industrialized one-step method for extracting housefly larvae fat, comprising the following steps of: (1) collecting larvaes, cleaning intestines, and removing remained feed with NaCl solution; (2) carrying out heat treatment to the larveaes for denaturation; (3) squeezing the larvaes and drying larvae slurry; (4) grinding the dried larvae slurry; and (5) extracting larvae fat from the larvae slurry powder by a supercritical CO2 extraction device, wherein the extract and solution are subjected to separation twice, i.e., separation I and separation II, and the conditions of the method are follows: extraction pressure is 20-35MPa, extraction temperature is 31-45 DEG C, extraction time is 1-4 hours, CO2 flux is 10-30kg / h, pressure of the separation I is 25-35MPa, separation I temperature is 29-45 DEG C, separation II pressure is 5-7MPa, and separation II temperature is 25-35 DEG C. In the method, harmful substances and impurities in fat are automatically removed, larvae fat with high quality is obtained in one step, and the method is suitable for extracting and purifying larvae fat from tens to tons grams of material and effectively solves the problem of industrialized extraction of housefly larvae fat.

Description

technical field [0001] The invention relates to a method for extracting housefly larvae, in particular to an industrialized one-step method for extracting pure housefly larvae. Background technique [0002] Unsaturated fatty acids account for 64.5% in the larval fat (insect fat) of the housefly (Musca domestica), and the P / S ratio is higher than that of most animal fats. Gas chromatography analysis of fatty acids (1999 Huazhong Agricultural University Journal Vol.18 No.3222-224), α-linolenic acid in housefly insect lipid is an essential fatty acid for human body to synthesize EPA and DHA. Therefore, insect fat has excellent health care functions and can be used in the fields of food, cosmetics and health care products. [0003] The traditional process of extracting and refining housefly insect lipid technology includes: harvesting larvae, removing impurities, washing, drying, crushing, leaching with volatile organic solvents (such as ether, petroleum ether or cyclohexane, e...

Claims

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Application Information

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IPC IPC(8): C11B1/02C11B1/10
Inventor 杜荣骞
Owner 本溪恒远生物医药科技有限公司
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