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Composition for treatment of joint disease

A composition and arthritic technology, applied in the direction of bone disease, drug combination, tissue regeneration, etc., can solve the joint disease of alginic acid composition without curing, and the therapeutic effect of alginic acid composition without cells is unknown. and other problems, to achieve the effect of inhibiting osteochondral destruction, improving joint function, inhibiting inflammatory response and pain

Inactive Publication Date: 2010-09-15
MOCHIDA PHARM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the therapeutic effect of the alginic acid composition that does not contain cells is unknown, and no attempt has been made to apply a non-curable alginic acid composition to joint diseases.

Method used

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  • Composition for treatment of joint disease
  • Composition for treatment of joint disease
  • Composition for treatment of joint disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0139] Rabbit Cartilage Repair Model

[0140] (1) Production of transplanted cells

[0141] To prepare cells for transplantation, bone marrow mesenchymal stromal cells (BMSC: Bone marrow mesechymal stromal cells) were isolated and cultured. BMSCs contain blood cell lineage cells (blood lineage cells) and the like in addition to bone marrow mesenchymal stem cells. 10 mL of bone marrow was collected from the tibia of 4-month-old Japanese white rabbits, washed twice with Ca·Mg-free PBS (Gibco BRL Lab.), suspended in DMEM-high glucose (DMED-HG, Sigma Chemical, St.Louis, MO). Blood clots were removed using a cell strainer (Falcon Co. Ltd) with a pore size of 70 μm. Cells were cultured in DMEM-HG, 10% fetal bovine serum (FBS, Gibco, Life Technology, Grand Island, NY), 1% antibiotic substance (Penicillin-Streptomycin-Fungizone 100X concentrated, Cambrex Biosciences, Walkersville, MD) , Use a 100mm Petri dish at 37°C, 5% CO 2 , and incubated under humidification. The medium ...

Embodiment 2

[0176] Molecular Weight Distribution Determination of Purified Sodium Alginate

[0177] (1) Method

[0178] The molecular weight distribution measurement of purified sodium alginate was carried out by gel filtration chromatography (Gel Filtration Chromatography) under the following conditions.

[0179] Column: TSKgel GMPWx1 2 pieces + TSKgel G2500PWx1 1 piece (manufactured by Tosoh Corporation)

[0180] (diameter 7.8mm×300mm×3 pieces)

[0181] Column temperature: 40°C

[0182] Eluent: 200mM sodium nitrate aqueous solution

[0183] Sample concentration: 0.05%

[0184] Flow rate: 1.0mL / min

[0185] Injection volume: 200μL

[0186] Detector: RI (differential refractometer)

[0187] Standard substances: pullulan, glucose (molecular weight 1.6 million, 788,000, 404,000, 212,000, 112,000, 47,300, 22,800, 11,800, 5900, 180)

[0188] (2) Results

[0189] [Table 1]

[0190] Test sample

mn

Number average molecular weight

mw

Weight aver...

Embodiment 3

[0194] Rabbit osteoarthritis model (anterior cruciate ligament (ACL) resection model)

[0195] (1) Method

[0196] Using female Japanese white rabbits (Japanese white rabbit; body weight 2.6-2.9kg), OA models were made for both knee joints according to the method of Vignon E et al. (Vignon E, Bejui J, Mathieu P, Hartmann JD, Ville G, Evreux JC, et al. Histological cartilage changes in a rabbit model of osteoarthritis. J Rheumatol 1987; 14(SpecNo): 104-6). Prepare 3 animals (6 knees) for each of the following 4 groups.

[0197] A) control group (administered with saline),

[0198] B) 1% sodium hyaluronate solution administration group (molecular weight is about 900,000, viscosity is about 2300mpa·s)

[0199] C) 1% purified sodium alginate solution administration group (molecular weight is about 1.7 million, viscosity is about 500mPa·s)

[0200] D) 2% purified sodium alginate solution administration group (molecular weight is about 1.7 million, viscosity is about 5000mPa...

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Abstract

Disclosed is a composition for treating a joint disease, which is characterized by comprising, as an active ingredient, a univalent metal salt of alginic acid which has an endotoxin level reduced to such an extent that an inflammation or attach of fever is not substantially induced. It becomes possible to provide a composition for treating a joint disease, which has an effect of protecting a cartilage from a mechanical stimulus, an effect of preventing the degeneration / deformation of a cartilage caused by friction or an inflammation, an effect of repairing a damaged part in an cartilage, an effect of preventing an inflammation or a pain in ajoint tissue, an effect of preventing the degeneration of a synovial tissue, and an effect of preventing the destruction of a bone or cartilage.

Description

technical field [0001] The present invention relates to a composition for treating joint diseases, including veterinary use and the like. Background technique [0002] Articular cartilage, for example, is hyaline cartilage and contains a small number of cells, a collagenous extracellular matrix, large amounts of proteoglycans, and water. In the case of bone, there are blood vessels and neural networks, and it has self-healing ability, so even if it is fractured, the fractured part can be fully repaired in many cases. However, blood vessels and nerve networks are absent in articular cartilage. Therefore, there is little self-repair ability, and especially in the case of forming a large cartilage defect, the cartilage defect cannot be sufficiently repaired. Even for the repaired part, fibrocartilage with different mechanical properties from hyaline cartilage is formed. Therefore, when cartilage defects are formed, arthralgia and loss of joint function are caused, often deve...

Claims

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Application Information

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IPC IPC(8): A61L27/00A61K31/734A61K35/28A61P19/02A61P29/00
CPCA61K31/734A61L2430/24A61L27/20A61P19/00A61P19/02A61P29/00C08L5/04
Inventor 岩崎伦政三浪明男笠原靖彦五十岚达弥河村太介大泽伸雄今井真理子
Owner MOCHIDA PHARM CO LTD
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