Nucleotide sequence for reinforcing antigenicity of listeria monocytogenes listeriolysin O

Abstract

The invention discloses a nucleotide sequence for reinforcing the antigenicity of listeria monocytogenes listeriolysin O. The antigenicity of the listeria monocytogenes listeriolysin O is reinforced by mutation, and a plasmid template-listeria monocytogenes listeriolysin O nucleotide sequence for mutation is prepared; and the plasmid template undergoes site-directed mutagenesis, and thus, the nucleotide gene sequence is prepared. The nucleotide gene sequence of the invention has the advantages of improving the antigenicity of the listeria monocytogenes listeriolysin O, obviously improving the detection sensitivity to the listeria monocytogenes in food and providing useful alternate gene resources for the development of a foodborne pathogen detection method.

Description

technical field The invention belongs to the field of genetic engineering, in particular to a nucleotide sequence for enhancing the antigenicity of listeria monocytolysin O. Background technique Agricultural products and food safety are a global problem, and foodborne diseases are the main problem of food safety. In recent years, food-borne disease incidents have occurred frequently at home and abroad, and Listeria monocytogenes plays a major role in food-borne bacterial poisoning. Listeria is the most important human food-borne pathogen, and it is one of the four major pathogens in food in the 1990s, and it is listed as one of the seven major food-borne pathogens in the United States. Listeria monocytogenes widely exists in nature, is not easy to be frozen and thawed, and can withstand high osmotic pressure. It can be found in soil, surface water, sewage, waste water, plants, silage feed, and rotten vegetables. , so animals can easily ingest the bacteria and spread it thr...

AI Technical Summary

Problems solved by technology

L. monocytogenes mutants that do not produce LLO can survive for some time in the cytosol of non-phagocytic cells, but cannot reproduce and cannot infect other cells because they cannot escape the phagosome

In addition, LLO is also involved in other reactions related to the pathogenicity of Listeria monocytogenes. Studies have shown that: a Listeria monocytogenes infection of dendritic cells in the spleen and bone marrow of mice can lead to cell apoptosis; L. monocytogenes mutants that do not produce LLO cannot induce apoptosis, whereas purified LLO can cause this programmed cell death

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