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Biological enzymolysis and purification method of high-quality stevioside

Inactive Publication Date: 2012-03-21
江苏健佳药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing stevioside extraction method has the following deficiencies: the one is that the extraction process cycle is long, the water consumption is large, and the sewage discharge is serious in the production process; High, low filtration efficiency, a large amount of protein, starch, tannin and other impurities are difficult to decompose or remove, seriously affecting the quality of stevioside finished products; Fourth, the content of rebaudioside A in the finished product is relatively low, and the content of rebaudioside A is hovering It is around 90%, and the residue of rebaudioside C is relatively high, so the finished product of stevioside has poor taste and impure taste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Put the dry stevia leaves into the soaking tank or other corresponding soaking containers, and add warm water 14 times the weight of the dry stevia leaves into the soaking tank, and the temperature of the warm water is 40°C; and add to the soaking liquid It is the acid-base regulator of 1% by weight of the stevia rebaudiana infusion, and the acid-base regulator is CaCl 2 , continuously stirring and soaking for 0.5h to form a stevia soaking solution with a pH of 4.5-5.0.

[0025] Activate the cellulase in warm water at 38°C-42°C for 5-10 minutes to activate the cellulase to restore its biological activity; at the same time, warm the stevia rebaudiana soaking solution with a pH value of 4.5-5.0 to 45°C. Adding activated cellulase into the warmed soaking liquid, keeping constant temperature and stirring continuously for 1 hour, the amount of cellulase added is 0.3%-0.5% of the dry weight of stevia rebaudiana. Add pectinase to the soaking liquid that has added cellulase an...

Embodiment 2

[0032] Put the dry stevia leaves into the soaking tank or other corresponding soaking containers, and add warm water 12 times the weight of the dry stevia leaves into the soaking tank, and the temperature of the warm water is 35°C; and add to the soaking liquid The stevia rebaudiana soaking liquid is 0.8% by weight of an acid-base regulator, and the acid-base regulator is sodium acetate, which is continuously stirred and soaked for 45 minutes to form a stevia rebaudiana soaking solution with a pH of 4.5-5.0.

[0033] Activate the cellulase in warm water at 38°C-42°C for 5-10 minutes to activate the cellulase to restore its biological activity; at the same time, warm the stevia rebaudiana soaking solution with a pH value of 4.5-5.0 to 40°C. Adding activated cellulase into the warmed soaking liquid, keeping constant temperature and stirring continuously for 1.25h, the amount of cellulase added is 0.3%-0.4% of the dry weight of stevia rebaudiana. Add pectinase to the soaking liqu...

Embodiment 3

[0040] Put the dry stevia leaves into the soaking tank or other corresponding soaking containers, and add warm water 15 times the weight of the dry stevia leaves into the soaking tank, and the temperature of the warm water is 45°C; and add to the soaking liquid The acid-base regulator is 1.2% by weight of the stevia rebaudiana soaking solution, and the acid-base regulator is magnesium chloride, which is continuously stirred and soaked for 1 hour to form a stevia rebaudiana rebaudiana soaking solution with a pH of 4.5-5.0.

[0041] Activate the cellulase in warm water at 38°C-42°C for 5-10 minutes to activate the cellulase to restore its biological activity; at the same time, warm the stevia rebaudiana soaking solution with a pH value of 4.5-5.0 to 50°C. Adding activated cellulase into the warmed soaking liquid, keeping constant temperature and stirring continuously for 1.5 hours, the amount of cellulase added is 0.4%-0.5% of the dry weight of stevia rebaudiana. Add pectinase t...

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Abstract

The invention discloses a biological enzymolysis and purification method of high-quality stevioside. The method comprises the following steps of: soaking dry stevioside leaves and regulating the pH value; sequentially adding cellulase and pectinase to the soak solution and filtering out leaf residues to obtain a filter clear solution; loading the filter clear solution into a macroporous absorption resin column to carry out column adsorption, and then eluting impurities in the macroporous absorption resin column by using water; analyzing effective constituents by using an analysis alcohol solution to prepare an alcohol analysis solution; adding active carbon to the stevioside alcohol analysis solution, and after filtering, sequentially leading the stevioside alcohol analysis solution to flow through a cation resin exchange column and an anion resin exchange column; concentrating and drying into a crude stevioside product; adding the alcohol solution to the crude stevioside product and naturally crystallizing the crude stevioside product; then filtering crystals and washing, drying, purifying and hydrolyzing the crystals by using absolute alcohol; and concentrating and drying into the high-quality stevioside. The process has reasonable flow and high extraction rate, the content of rebaudioside-A is higher than 98 percent, and the taste of the finished product is pure.

Description

technical field [0001] The invention belongs to the technical field of food additives, and relates to a method for extracting natural high-efficiency sweeteners, in particular to a production method for extracting stevioside containing more than 98% rebaudioside A from herb stevia rebaudiana through biological enzymatic hydrolysis. Background technique [0002] Stevioside is a natural sweetener extracted from the Compositae herb stevia. It has the characteristics of high sweetness and low calorie. Its sweetness is 200-300 times that of sucrose, and its calorific value is only 1 / 300 of sucrose. A large number of drug experiments have proved that stevioside has no toxic side effects and is safe to eat. Regular consumption can prevent high blood pressure, diabetes, obesity, heart disease and other diseases. It is a natural sweetener with great development value and health praise. It is an alternative Sucrose is an ideal sweetener, which is widely used in food, beverage, medicin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14
Inventor 周勇徐柏衡
Owner 江苏健佳药业有限公司
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