Biological enzymolysis and purification method of high-quality stevioside

Abstract

The invention discloses a biological enzymolysis and purification method of high-quality stevioside. The method comprises the following steps of: soaking dry stevioside leaves and regulating the pH value; sequentially adding cellulase and pectinase to the soak solution and filtering out leaf residues to obtain a filter clear solution; loading the filter clear solution into a macroporous absorption resin column to carry out column adsorption, and then eluting impurities in the macroporous absorption resin column by using water; analyzing effective constituents by using an analysis alcohol solution to prepare an alcohol analysis solution; adding active carbon to the stevioside alcohol analysis solution, and after filtering, sequentially leading the stevioside alcohol analysis solution to flow through a cation resin exchange column and an anion resin exchange column; concentrating and drying into a crude stevioside product; adding the alcohol solution to the crude stevioside product and naturally crystallizing the crude stevioside product; then filtering crystals and washing, drying, purifying and hydrolyzing the crystals by using absolute alcohol; and concentrating and drying into the high-quality stevioside. The process has reasonable flow and high extraction rate, the content of rebaudioside-A is higher than 98 percent, and the taste of the finished product is pure.

Description

technical field [0001] The invention belongs to the technical field of food additives, and relates to a method for extracting natural high-efficiency sweeteners, in particular to a production method for extracting stevioside containing more than 98% rebaudioside A from herb stevia rebaudiana through biological enzymatic hydrolysis. Background technique [0002] Stevioside is a natural sweetener extracted from the Compositae herb stevia. It has the characteristics of high sweetness and low calorie. Its sweetness is 200-300 times that of sucrose, and its calorific value is only 1 / 300 of sucrose. A large number of drug experiments have proved that stevioside has no toxic side effects and is safe to eat. Regular consumption can prevent high blood pressure, diabetes, obesity, heart disease and other diseases. It is a natural sweetener with great development value and health praise. It is an alternative Sucrose is an ideal sweetener, which is widely used in food, beverage, medicin...

AI Technical Summary

Problems solved by technology

The existing stevioside extraction method has the following deficiencies: the one is that the extraction process cycle is long, the water consumption is large, and the sewage discharge is serious in the production process; High, low filtration efficiency, a large amount of protein, starch, tannin and other impurities are difficult to decompose or remove, seriously affecting the quality of stevioside finished products; Fourth, the content of rebaudioside A in the finished product is relatively low, and the content of rebaudioside A is hovering It is around 90%, and the residue of rebaudioside C is relatively high, so the finished product of stevioside has poor taste and impure taste