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Vinegar residue fermented complex micro organism fungicide and preparation method thereof

A technology of composite microbial agent and vinegar grains, which is applied in the field of microbial technology treatment of organic waste agricultural resources, can solve the problems of long cycle and difficult fermentation, achieve strong acid resistance, accelerate the fermentation process, and shorten the fermentation cycle.

Inactive Publication Date: 2010-08-18
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the technical difficulties of difficult fermentation and long period of the existing raw material of fresh vinegar lees, and to provide a fermentation bacterial agent that can speed up fermentation and increase the nutrient content of fermentation products and its preparation method

Method used

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  • Vinegar residue fermented complex micro organism fungicide and preparation method thereof

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Embodiment Construction

[0017] 1. Screening of single strains fermented with vinegar lees

[0018] In order to ensure that the microorganisms that are really suitable for the raw material of vinegar grains are screened, the preliminary screening is carried out from the fresh vinegar grains themselves and naturally fermented raw materials of vinegar grains. Fresh vinegar grains and fermented vinegar grains were collected respectively, streaked and inoculated on TSA medium and PDA culture based on the concentration dilution method and cultured at 28°C. Through multiple isolations, 13 typical single colonies were preliminarily obtained; through solid primary screening culture Base (CMC plate culture medium) and the mensuration of CMC cellulase activity (both common methods) under the culture of liquid fermentation medium, according to the intensity of decomposing CMC cellulose ability of isolated bacterial strain, further screening is carried out to isolated bacterial strain, through After multiple pass...

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Abstract

The invention discloses a preparation method of vinegar residue fermented complex micro organism fungicide. The fungicide is formed by single strain fungicides of bacillusacidocaldarius., rhizopus stolonifer and trichoderma viride by weight ratio of 1:1:1, wherein firstly stock seeds of the single strain fungicides are respectively multiplied by a primary test tube and are respectively subject to secondary liquid culture to obtain bacteria; then 50-60% of rice bran and 50-40% of wheat bran are mixed by weight percent and stirring to be uniform is carried out to obtain mixed material, the cultured bacteria are respectively inoculated according to the volume weight ratio of bacterium and mixed material, thus obtaining single strain fungicide. The complex micro organism fungicide provided by the method can increase fermentation temperature of vinegar residue compost and speed up fermentation, and fermentation finished product thereof has high content of full dose nutrients (nitrogen, phosphorous and potassium) and good nutrition.

Description

technical field [0001] The invention belongs to the field of microbial technology treatment of organic waste for agricultural use, and relates to a compound microbial bacterial agent capable of promoting the fermentation of vinegar grains and a preparation method thereof. Background technique [0002] Vinegar grains are the leftovers of vinegar factories after soaking in vinegar. Due to strong acidity, high water content and a large amount of crude fiber (rice husk), they have not been well developed and utilized for a long time. These leftovers have been discarded and piled up for many years. Pollution of the environment also restricts the development of the enterprise itself. Therefore, how to deal with and utilize it is a major problem faced by the vinegar industry at present, and it is also one of the priorities of the local environmental protection department. [0003] At present, except for a small amount of fresh vinegar residues that are sold directly, most of the v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12N1/14C05F17/00C12R1/07C12R1/845C12R1/885
CPCY02W30/40
Inventor 朱咏莉李萍萍吴沿友杨运克孙德民
Owner JIANGSU UNIV
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