Method for preparing colon cancer chemotherapy medicament
A chemotherapeutic drug and colon cancer technology, applied in the field of preparation of colon cancer chemotherapeutics, can solve the problems of reducing human immune function, toxic and side effects, affecting chemotherapeutic drugs, etc.
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[0017] The preparation method of the chemotherapy colon cancer compound agent of the present invention, its preparation steps are as follows:
[0018] 1) Preparation of low-density lipoprotein:
[0019] (1) Preparation of KBr-NaEDPA solution: Weigh 1.080 g of KBr, add 0.0022 mol / L NaEDPA 100 ml aqueous solution, dissolve and stir evenly for later use.
[0020] (2) According to the blood type of the plasma raw material stored in the blood bank, take 200ml of plasma respectively, centrifuge at 300r / m for 10 minutes, take the supernatant, adjust the density to 1.01ρ with KBr-NaEDPA solution, control the temperature at 4°C, and centrifuge at 47000r Centrifuge for 18 hours at a speed of 1 / m, suck off the VLDL solution in the upper layer, and leave the remaining part for later use;
[0021] (3) Mix the remaining liquid after absorbing the VLDL in the upper layer, then use KBr-NaEDPA solution to adjust the density to 1.063ρ, control the temperature at 4°C, centrifuge at 47000r / m for...
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