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Method for extracting high-efficiency bezafibrate degrading bacteria

A technology for efficiently degrading bacteria and bezafibrate, applied in the field of separation and purification of microbial bacteria, can solve the problems of ineffective removal, frequent detection, and high detection concentration, and achieve low cost, easy separation and purification methods, and environmental protection. friendly effect

Inactive Publication Date: 2010-08-04
DONGHUA UNIV
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Problems solved by technology

Therefore, it is particularly important to control bezafibrate in natural water bodies. However, bezafibrate is a nitrogen-containing aromatic heterocyclic compound. At present, traditional sewage treatment methods (such as adsorption, activated sludge, biofilm, ultraviolet light, photocatalytic degradation, etc.) method) cannot be effectively removed, so that it is frequently detected and the detection concentration is relatively high

Method used

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  • Method for extracting high-efficiency bezafibrate degrading bacteria
  • Method for extracting high-efficiency bezafibrate degrading bacteria
  • Method for extracting high-efficiency bezafibrate degrading bacteria

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Embodiment 1

[0035] (1) The activated sludge taken from the secondary settling tank of the urban sewage treatment plant is inoculated in the inorganic salt medium by 5% of the access amount, wherein the inorganic salt medium is K 2 HPO 4 4.35g, KH 2 PO 4 1.7g, NH 4 Cl 2.1g, MgSO 4 0.2g, MnSO 4 0.05g, FeSO 4 ·7H 2 O 0.01g, CaCl 2 2H 2 O 0.03g, distilled water 1000mL, pH=6.8-7.0. Acclimate by gradient pressure acclimatization method, the concentration of bezafibrate is 10, 15, 20, 25, 30, 35mg / L, at 30℃, 160r / min, and the filling volume of 250mL Erlenmeyer flask is 100mL. , the total domestication time is 2 months;

[0036] (2) Take the sample after 2 months of acclimation, let it stand for 30 minutes, take 1mL of the upper layer water sample, and make 10 -1 ~10 -10 Gradient dilution. For each dilution concentration, take 1mL bacterial solution in a petri dish, pour the solid medium into it, put the petri dish on the table, turn the petri dish back and forth, put the petri d...

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Abstract

The invention relates to a method for extracting high-efficiency bezafibrate degrading bacteria, which comprises the following steps: (1) inoculating activated sludge from a secondary sedimentation tank of an urban sewage treatment plant to an inorganic salt culture medium in an inoculation amount of 5 weight percent and domesticating for 2 months in a gradient pressure domestication way; and (2) standing a sample domesticated for 2 months for 30 minutes, taking 1mL of supernatant water sample for gradient dilution, inoculating 1mL of bacterial solution with each dilution concentration respectively to a solid culture medium for enrichment culture to ensure that a pure single strain is grown, and inoculating the pure single strain to a slant culture medium and storing at the temperature of 4 DEG C. In the method, the bezafibrate degrading bacterial strains are extracted from the activated sludge in the secondary sedimentation tank of the urban sewage treatment plant, so the bezafibrate degrading bacterial strains have the advantages of readily available source, simple and convenient separation and purification method, low cost and environmental protection; and the extracted bacterial strains have a bezafibrate degrading rate of over 85 percent and have good application prospect.

Description

technical field [0001] The invention belongs to the field of separation and purification of microbial bacteria, and in particular relates to an extraction method of bezafibrate highly efficient degrading bacteria. Background technique [0002] With the improvement of environmental analysis technology and the enhancement of people's environmental awareness, Pharmaceutical and Personal Care Products (PPCPs), as a new pollutant, has been paid more and more attention by people. Drugs and personal care products still have a considerable degree of biological activity even if they exist in trace amounts, causing unpredictable impacts on the environment, and may cause potential harm to human health through drinking water and food. [0003] Among the common PPCPs compounds, antibiotics, anti-inflammatory drugs, antiepileptic drugs, and blood lipid regulating drugs were frequently detected in various environmental media. Due to the improvement of living standards, Bezafibrate (bezafi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/38
Inventor 薛罡杨林刘亚男龚清杰高品史锦程起跃周宁娟王志楼
Owner DONGHUA UNIV
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