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Method for predicting staphylococcus aureus growth and toxin production in milk

A staphylococcus and aureus technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of long detection time and complicated operation, and achieve high accuracy, simple operation, and time-saving effects

Inactive Publication Date: 2010-07-28
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] The present invention aims to solve the problems of complex operation and long detection time of the existing methods for detecting the number of Staphylococcus aureus and Staphylococcus aureus enterotoxin in milk, and provides a method for predicting the growth and toxin production of Staphylococcus aureus in milk Methods

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  • Method for predicting staphylococcus aureus growth and toxin production in milk
  • Method for predicting staphylococcus aureus growth and toxin production in milk
  • Method for predicting staphylococcus aureus growth and toxin production in milk

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specific Embodiment approach 1

[0012] Embodiment 1: The method for predicting the growth and toxin production of Staphylococcus aureus in milk in this embodiment is carried out according to the following steps: detecting the growth temperature of Staphylococcus aureus in milk and the initial bacterial count of Staphylococcus aureus in milk, and then The temperature of the sample to be tested is substituted into y=7.1917×10 -5 ×T 2 +0.022782×T-0.32889 formula, calculate the maximum growth rate of Staphylococcus aureus in milk, wherein T represents the growth temperature of Staphylococcus aureus, and y represents the maximum growth rate of Staphylococcus aureus in milk; 2. Step 1 The initial bacterial count of Staphylococcus aureus in middle milk and the maximum growth rate of Staphylococcus aureus in milk are substituted into the formula log N(t)=A+C[exp(-exp(-y(t-M)))], Calculate the number of Staphylococcus aureus in milk at time t, where exp=e x , t represents the growth time of Staphylococcus aureus in...

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Abstract

The invention discloses a method for predicting staphylococcus aureus growth and toxin production in milk, which relates to a method for predicting staphylococcus aureus growth and toxin production through the principle of predictive microbiology. The invention solves the problems that the method for testing the number of staphylococcus aureus and staphylococcus aureus enterotoxin in milk is complex to operate, and the test time is long. In the method: first, the maximum growth rate is obtained according to 7.1917 * 10-5* T2 +0.022782* T-0.32889; second, the bacterial count is obtained according to log N (t) = A + C (exp (-exp (-y (t-M)))); third, the time corresponding to the bacterial count 106.4cfu / mL is combined with F=0.0018543 * T2-0.050277* T +0.32629 to obtain the toxin production speed rate, that is, the prediction of the staphylococcus aureus growth and toxin production is realized. The invention has the advantages of simple operation and short test time.

Description

technical field [0001] The invention relates to a method for predicting the growth and toxin production of Staphylococcus aureus. Background technique [0002] At present, there are mainly two detection methods for the presence or absence of Staphylococcus aureus in milk, namely, the national standard plate count method and PCR detection method, but both methods are to determine the presence of Staphylococcus aureus in the sample at the time point of sampling. For the number of cocci, if you want to know the number of Staphylococcus aureus at other time points in the sample, you need to repeat the operation, which is complicated. The existing method for determining Staphylococcus aureus enterotoxin is mainly the ELISA method and the detection kit based on this method. Although the sensitivity of the detection kit is very good, it can only give qualitative or semi-quantitative results, which will cause The harmfulness of milk cannot be accurately assessed. Contents of the ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14C12Q1/06
Inventor 姜毓君赵凤曲行光吕学娜薛玉清谢鲲昊杨士芹姚丽燕
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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