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Method and device for optimizing and scaling up vitamin B12 fermentation process

A fermentation process and sensing device technology, applied in microorganism-based methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problem of lack of optimization and amplification methods, and no characteristic parameters of cell metabolite flow distribution changes. And other issues

Inactive Publication Date: 2010-07-14
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] However, the shortcomings of the above-mentioned existing amplification criteria are that they are all based on the static operation method based on the optimal process control point based on the detection of extracellular parameters, and do not focus on the relevant phenomena based on the change of the distribution of the flow of the metabolic substance in the cell. Characteristic Parameters
[0020] In summary, the lack of vitamin B in this field 12 Method for optimization and scale-up of fermentation process, and vitamin B 12 Industrial-scale bioreactors for fermentation

Method used

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  • Method and device for optimizing and scaling up vitamin B12 fermentation process
  • Method and device for optimizing and scaling up vitamin B12 fermentation process
  • Method and device for optimizing and scaling up vitamin B12 fermentation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Example 1: B 12 Fermentation process optimization and scale-up

[0117] 1 Materials and methods

[0118] 1.1 Strain and culture medium

[0119] 1.1.1 Bacterial strain: Pseudomonas denitrificans, provided by Shijiazhuang Pharmaceutical Group Huarong Pharmaceutical Co., Ltd.

[0120] 1.1.2 Medium

[0121] Primary seed medium: molasses, corn steep liquor, KH 2 PO 4 , (NH 4 ) 2 SO 4 , (NH 4 ) 2 HPO 4 , MnSO 4 Wait.

[0122] Secondary seed media: molasses, corn steep liquor, KH 2 PO 4 , (NH 4 ) 2 SO 4 , (NH 4 ) 2 HPO 4 , MgSO 4 Wait.

[0123] Fermentation medium: molasses, sucrose, betaine, (NH 4 ) 2 SO 4 , MgSO 4 、CoCl 2 , DMBI, ZnSO 4 Wait.

[0124] 1.2 Fermentation tank: the volume of the pilot test fermentation tank is 9m 3 , the agitator is axial; the volume of the industrial scale fermentation tank is 120m 3 , the main flow of the agitator is axial mixing.

[0125] 1.3 Analysis method

[0126] 1.3.1 Biomass measurement: measure the dry ...

Embodiment 2

[0162] Such as Figure 6 Shown, self-control bioreactor device of the present invention comprises the bioreactor tank body (100) that has stirrer (104), and this device is by the fermenter tank body of belt motor and stirrer (104), has multi-parameter detection and It consists of a sensor system for controlled instruments and sensors, a mounting bracket and a process piping system, and an electrical control cabinet with an industrial computer and executive components, wherein the sensor system includes a temperature sensor (1), a pH Sensor(2), Dissolved Oxygen Sensor(3), Full Tank Scale Sensor(4), Exhaust Gas CO 2 Port (5), exhaust gas O 2 Interface (6), velocity sensor (7), pressure sensor (8), defoaming sensor (9), ventilation flow sensor (26), live cell mass sensor (10); Described parameter refers to temperature, stirring speed, Ventilation flow, tank pressure, defoaming, pH, dissolved oxygen concentration, real volume and weight of fermentation broth, feeding amount incl...

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Abstract

The invention provides a method for optimizing and scaling up the fermentation process using a bioreactor device. The method comprises the following steps: 1. measuring physiological parameters and the related characteristics or combined characteristics of the physiological parameters of the fermentation process of the bioreactor device, wherein the physiological parameters are selected from the following parameters: dissolved oxygen, oxygen uptake rate, pH, carbon dioxide excretion rate, respiratory quotient, living cell rate or cell shape, consumption of measured metabolic product or matrix or combined characteristics; and 2. comparing the physiological parameters and the related characteristics or combined characteristics which are measured in the step 2, with preset physiological parameters and the related characteristics or combined characteristics; selecting a bioreactor device of which data are most similar to the preset values; and determining the optimized and scaled-up bioreactor device, wherein the physiological parameters are shown in the step 1.

Description

technical field [0001] The invention relates to a method for optimizing and enlarging a fermentation process and its device, in particular to a vitamin B 12 A method and device for optimizing and amplifying a fermentation process. Background technique [0002] In order to make the rules and data obtained from small-scale fermentation experiments reproduced in large-scale production, it is necessary to master and use amplification technology. In the prior art, a similar principle is generally used for comparison and amplification. Specifically, the basic method of comparison and amplification in the prior art is: firstly, it is necessary to find out various parameters characterizing the system, compose them into several dimensionless numbers with certain physical meanings, and establish the functional formula between them, and then The constants and exponents contained in this functional formula are obtained in the test equipment by experimental methods, then this relationa...

Claims

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Application Information

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IPC IPC(8): C12P19/42C12M1/38C12M1/36C12M1/34C12M1/02C12R1/38
CPCC12M41/32C12M41/26
Inventor 庄英萍张嗣良李昆太刘东洪储炬王永红黄明志杭海峰
Owner EAST CHINA UNIV OF SCI & TECH
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